Method for preparing bile salt hydrolase lactobacillus powder starter

Abstract

The invention relates to a method for preparing a bile salt hydrolase lactobacillus powder starter, which is suitable for producing powder starters for fermented dairy products such as functional yoghourt and probiotics preparations. The method comprises the following steps of: performing activation and amplification culture on Lactobacillus casei KL1, Lactococcus lactis lactis KS4 and Streptococcus thermophiles Tx, which are screened from Tibetan kefir and are strains of bile salt hydrolase; performing high density culture by using an automatic fermentation tank; performing centrifugal concentration and adding a freeze-drying protective agent for prefreezing and freeze drying to prepare the bile salt hydrolase lactobacillus powder starter. After the powder starter prepared by the method is used for fermenting milk, the obtained yoghourt has sticky, fine and smooth mouthfeel, moderate sweet and sour taste, and thick ester aroma.

Description

technical field [0001] The invention relates to a preparation method of a dry powder starter of lactic acid bacteria producing bile salt hydrolyzing enzyme, which is suitable for the production of a dry powder starter of fermented dairy products such as functional yogurt and probiotic preparations. Background technique [0002] Kefir is a traditional alcoholic fermented milk drink made from cow's milk or goat's milk, added with kefir grain starter containing lactic acid bacteria and yeast, and fermented. The kefir milk product of industrialized production is to be raw material with cow's milk at present, utilizes the lactobacillus and the saccharomycete that are separated from kefir grain (claiming Tibetan spirit mushroom again) to carry out fermentation and make. Kefir originated in the North Caucasus region of Russia. Local herdsmen poured cow's milk or goat's milk into sheepskin pockets for natural fermentation to produce yoghurt. The residue was added with cow's or goat'...

AI Technical Summary

Problems solved by technology

However, there are no relevant literature reports and patent reports at home and abroad about the method of separating and screening the lactic acid bacteria producing bile salt hydrolase from the mushroom, using automatic fermenters for high-density culture, and preparing dry powder starter.

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