Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof

A technology of organic waste and bacterial agent, applied in the biological field, can solve the problems of reduced decomposition ability of organic waste, high requirements for fermentation conditions, and unstable effect

Active Publication Date: 2013-09-25
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Purified bacteria with a single bacterial agent have a narrow range of adaptation to growth conditions, high requirements on fermentation conditions, and are easily polluted and inhibited by other bacteria; due to the loss of the coexistence of other bacteria that have long been in a synergistic relationship under natural conditions, their organic waste The decomposition ability of the substance is greatly reduced
The strains contained in the natural fermented product bacterial agent are a multi-bacteria consortium assembled disorderly in the natural passive fermentation process. The fermentation process of the bacterial agent follows the natural circulation law, its decomposition efficiency is low, and the effect is unstable

Method used

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  • Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, preparation and detection of composite bacterial agent

[0048] 1. Bacteria culture on inclined plane

[0049] The preservation number of Sporotrichum thermophile is CICC2441, the preservation number of Trichoderma konigii is CGMCC3.4001, the preservation number of Phanerochaete chrysosporium is CGMCC5.776, and the preservation number of Aspergillus niger is CGMCC3. 03147, Saccharomyces cerevisiae (Saccharomyces cerevisiae) preservation number CICC1415, Bacillus subtilis (Bacillus subtilis) preservation number CICC10076, and Streptomyces ambofaciens (Streptomyces ambofaciens) preservation number ACCC40133. It was cultured statically for 48 hours at a temperature of 26°C.

[0050] 2. Expanded cultivation of liquid seeds

[0051] The strains cultured on the slant were picked and inoculated in the corresponding liquid medium (1L medium was inoculated with 4-6 needles), cultured on a shaking table at 28°C for 72 hours at a speed of 200rpm, and seven strains ...

Embodiment 2

[0061] Embodiment 2, preparation and detection of composite bacterial agent

[0062] 1. Bacteria culture on inclined plane

[0063] The preservation number of Sporotrichum thermophile is CICC2441, the preservation number of Trichoderma konigii is CGMCC3.4001, the preservation number of Phanerochaete chrysosporium is CGMCC5.776, and the preservation number of Aspergillus niger is CGMCC3. 03147, Saccharomyces cerevisiae (Saccharomyces cerevisiae) preservation number CICC1415, Bacillus subtilis (Bacillus subtilis) preservation number CICC10076, and Streptomyces ambofaciens (Streptomyces ambofaciens) preservation number ACCC40133, these 7 strains were inoculated on the corresponding slant culture medium respectively. The cells were cultured statically for 24 hours at a temperature of 30°C.

[0064] 2. Expanded cultivation of liquid seeds

[0065] Pick the strains cultured on the slant and inoculate them respectively (4-6 needles in 1L culture medium) in the corresponding liquid ...

Embodiment 3

[0075] Embodiment 3, compound bacterial agent application

[0076] The compost with cassava residue as the main raw material is as follows:

[0077] Compost raw material composition: cassava alcohol residue (particle size less than 2mm, C / N about 45) 75% (mass percentage content) and filter mud (cassava alcohol plant waste water filter mud) 25% (mass percentage content);

[0078] Production process: Add the composite bacterial agent obtained in Example 1 or Example 2 respectively according to 0.2% and 0.22% of the composting raw material quality, adjust the C / N to 30 with urea, and adjust the water content to 60% (mass percentage content) , fully stirred evenly, and piled into strips with a height of no more than 80cm and a width of 150-200cm at the bottom. Manual turning is adopted, and the compost is turned once every 3 days during the high temperature period, and once every 5 days during the cooling period, to obtain fertilizer. The same compost raw material without compo...

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Abstract

The invention discloses a composite inoculant for promoting the decomposing of cellulose organic wastes, and a preparation method thereof. The active components of the inoculant for promoting the decomposing of cellulose organic wastes comprise Sporotrichum thermophile, Trichoderma konigii, Phanerochaete chrysosporium, Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Streptomyces ambofaciens. Experiments in the invention prove that the composite inoculant for promoting the decomposing of cellulose organic wastes can effectively improve the decomposing speed of the organic wastes, and can be used for producing biological organic fertilizers.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a composite bacterial agent for promoting the decomposition of organic waste and a preparation method thereof. Background technique [0002] Agricultural production, food, ethanol extraction and other light industries produce a huge amount of organic waste such as crop straw, vinegar residue, wine residue, etc. Most of these wastes are rich in cellulose, lignin and other refractory substances, which lead to the utilization The rate is not high, and most of them are treated as "garbage", which not only pollutes the environment but also causes waste of resources. At present, most of the bacteria agents commonly used to treat organic waste at home and abroad are purified single bacteria agents and naturally fermented bacteria agents. Purified bacteria with a single bacterial agent have a narrow range of adaptation to growth conditions, high requirements on fermentation conditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F11/08C05F17/00C12R1/885C12R1/685C12R1/865C12R1/125C12R1/465C12R1/645
CPCY02W30/40
Inventor 侯宪文郑鹏李勤奋邹雨坤李光义
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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