Lactic acid bacteria powder for suckling pigs as well as preparation method and using method thereof

A technology of lactic acid bacteria powder and lactic acid bacteria, which can be used in applications, animal feed, animal feed, etc., can solve the problems of limited application, high temperature resistance, etc., and achieve the effects of weight gain, hair shine, and mortality reduction

Active Publication Date: 2014-03-05
湖北绿天地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the use of microbial feed additives is mainly granulated into granulated feed or used in fermented f

Method used

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  • Lactic acid bacteria powder for suckling pigs as well as preparation method and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of Enterococcus faecalis powder

[0031] (1) Cultivation of Enterococcus faecalis seeds:

[0032] Inoculate Enterococcus faecalis into MRS medium (peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, K 2 HPO 4 2.0g, triammonium citrate 2.0g, sodium acetate 5.0g, MgSO 4 ·7H 2 O0.58g, MnSO 4 4H 2 O0.25g, Tween-801.0ml, pure water 1000ml, pH6.4-6.8, sterilized by high pressure steam at 121℃ for 15min). The cultivation temperature is 37±1°C, the cultivation time is 10h-18h, and the seed liquid is obtained.

[0033] (2) Fermentation culture of Enterococcus faecalis:

[0034] Inoculate the seed solution into the fermentation medium (peptone 3.6kg, yeast extract 3.0kg, glucose 4.0kg, magnesium sulfate 0.06kg, triammonium citrate 1.2kg, sodium acetate 1.8kg, tap water 600L, pH6.2-6.4, 121℃ Autoclave for 15 minutes). The culture temperature is 37±1℃, the culture time is 10h-18h, and the fermentation liquid is obtained

[0035] (3) After ...

Embodiment 2

[0048] 1. Preparation of binary compound powder of Enterococcus faecium and Lactobacillus casei

[0049] (1) Preparation of Enterococcus faecium powder:

[0050] The preparation of Enterococcus faecium powder is the same as the preparation of Enterococcus faecalis powder in Example 1;

[0051] The effective viable count of the collected Enterococcus faecium powder product is 48 billion / g, and the moisture content is 6.3%.

[0052] (2) Preparation of Lactobacillus casei powder:

[0053] 1) Cultivation of Lactobacillus casei seeds:

[0054] Lactobacillus casei was inoculated in the MRS medium (same as Example 1). The cultivation temperature is 35±2°C, the cultivation time is 12h-20h, and the seed liquid is obtained.

[0055] 2) Fermentation culture of Lactobacillus casei:

[0056]Inoculate the seed liquid into the fermentation medium (peptone 3.6kg, yeast extract 2.4kg, beef extract 0.6kg, glucose 3.2kg, magnesium sulfate 0.06kg, triammonium citrate 1.2kg, sodium acetate 1....

Embodiment 3

[0070] 1. Preparation of ternary compound powder of Enterococcus faecalis, Lactobacillus plantarum and Lactobacillus casei

[0071] (1) The preparation of Enterococcus faecalis is the same as in Example 1.

[0072] (2) The preparation of Lactobacillus casei is the same as in Example 2.

[0073] (3) Preparation of Lactobacillus plantarum powder

[0074] 1) Cultivation of Lactobacillus plantarum seeds:

[0075] Inoculate Lactobacillus plantarum into MRS medium (same as Example 1). The cultivation temperature is 35±2°C, the cultivation time is 12h-20h, and the seed liquid is obtained.

[0076] 2) Fermentation culture of Lactobacillus plantarum:

[0077] Inoculate the seed solution into the fermentation medium (peptone 4.2kg, yeast extract 3.0kg, glucose 3.6kg, magnesium sulfate 0.06kg, triammonium citrate 0.6kg, sodium acetate 1.2kg, tap water 600L, pH5.8-6.4, 121°C Autoclave for 15 minutes). The culture temperature is 35±1℃, the culture time is 12h-16h, and the fermentatio...

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Abstract

The invention provides a lactic acid bacteria powder for suckling pigs as well as a preparation method and a using method thereof and belongs to the technical field of microorganism feed additives. The adopted lactic acid bacteria comprise one or more of enterococcus faecalis, enterococcus faecium, pediococcus acidilactici, streptococcus thermophiles, lactobacillus acidophilus, lactobacillus plantarum and lactobacillus casei. The active viable count is 50-1000 hundred million/gram and the water content is 4-8%. When the lactic acid bacteria are ingested by newborn suckling pigs through the way spraying the lactic acid bacteria powder obtained by using the preparation method in mouth cavities of the newborn suckling pigs or on nipples of sows, the death rate of the suckling pigs is reduced; the rate of sick pigs with red or white dysentery is reduced; the weight is increased; the suckling pigs are vivacious and bright in fur; the special effects of the lactic acid bacteria are fully developed.

Description

technical field [0001] The invention belongs to the technical field of microbial feed additives, in particular to a lactic acid bacteria powder for suckling pigs and a preparation method and application method thereof. Background technique [0002] Since the 20th century, a variety of antibiotics and hormones have been widely used as feed additives. Although they have played an indispensable role in livestock and poultry production, disease prevention and promotion of animal growth, due to long-term use and even abuse, the use of antibiotics There are more and more problems in the use, mainly in: drug residues and toxicity; large-scale use of broad-spectrum antibiotics in feeding livestock causes rapid growth of bacterial resistance; long-term use of antibiotics disrupts the microbial community in the microbial system of livestock and poultry Micro-ecological balance, leading to digestive disorders, causing various digestive tract diseases; reducing the immune function of an...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16A23K1/18
Inventor 杨国军林开春江艳
Owner 湖北绿天地生物科技有限公司
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