Agricultural microbial fertilizer and preparation method and application thereof
A microbial fertilizer and bacillus technology, applied in the field of microorganisms, can solve the problems of treating the symptoms but not the root cause, not being widely used, and small treatment area, and achieving the effects of increasing the content of amino acids, increasing the stress resistance of crops, and enhancing soil fertility.
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Embodiment 1
[0023] This embodiment provides an agricultural microbial fertilizer and a preparation method thereof.
[0024] 1. Adopt nutrient gravy medium to cultivate Bacillus licheniformis and Bacillus subtilis respectively; Taking the total weight of this medium as a basis, the percentage composition of peptone in this medium can be 0.5%, the percentage composition of NaCl can be 0.5%, The percentage composition of beef extract can be 0.2%, surplus is sterile water, and the pH value of this culture medium is 7.0; Culture method is conventional culture method, cultivates until the number of viable bacteria of bacillus licheniformis is (1~10) ×10 10 CFU / g, the number of live bacteria of Bacillus subtilis is (1~10)×10 10 CFU / g.
[0025] 2, adopt YPG culture medium to cultivate Bacillus coagulans; Taking the gross weight of this medium as a basis, the percentage composition of peptone in this medium is 0.25%, the percentage composition of glucose is 0.25%, and the percentage composition ...
Embodiment 2
[0036] This example is used to illustrate the fertilization method and fertilization effect of the agricultural microbial bacterial fertilizer provided by the present invention for soil. The soil selected in this embodiment is paddy soil, and the experimental site is Jiayuan Agricultural Development Co., Ltd., Nankou Town, Meixian County, Meizhou City, Guangdong Province. The plant of the experiment is Sijiu Caixin. The experimental unit is commissioned by the General Station of Fertilizers in Guangdong Province.
[0037] Experimental method: Wherein the experimental group: the agricultural microbial bacterial fertilizer prepared in Example 1 was inoculated in the soil, the mass ratio of the agricultural microbial bacterial fertilizer to the soil was 0.2:100, stirred evenly and then fermented, the fermentation temperature was 20 ℃, the fermentation time is 5 days, and the fertilized soil is obtained. Then plant Sijiu Chinese cabbage in the fertilized soil, the planting area is...
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