Composite microbial fertilizer containing polyglutamate high-producing strain, preparation method and application thereof

A technology of compound microorganisms and polyglutamic acid, applied in application, fertilizer mixture, fertilization equipment, etc., can solve the problem of adding microbial fertilizers with high-yield polyglutamic acid strains that have not yet appeared

Active Publication Date: 2013-07-17
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research and development of compound microbial fertilizer has become a trend. In recent years, patents on compound microbial fertilizer are also common, but there is no invention of microbial fertilizer added with polyglutamic acid high-yielding strains.

Method used

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  • Composite microbial fertilizer containing polyglutamate high-producing strain, preparation method and application thereof
  • Composite microbial fertilizer containing polyglutamate high-producing strain, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Put the Bacillus licheniformis seed solution into the fermentation medium, 37°C, rotation speed 300rpm, ventilation ratio 1:1, and ferment for 24-48 hours to obtain the fermentation medium. The composition of the fermentation medium is: by mass percentage, 2% Sucrose, 0.4% beef extract, 2% monosodium glutamate, 1.2% MgSO 4 ·7H 2 0.0.06%K 2 HPO 4 ·3H 2 O, the rest is water, pH7.5;

[0044] The seed liquids of Bacillus megaterium, Bacillus thuringiensis, and Bacillus amyloliquefaciens were respectively inserted into the fermentation medium, and fermented for 20 hours at 37°C, with a rotation speed of 300 rpm and a ventilation ratio of 1:1, to obtain a fermentation liquid. The composition of the fermentation medium was : In terms of mass percentage, 1% glucose, 0.6% dipotassium hydrogen phosphate, 0.3% potassium dihydrogen phosphate, 0.15% urea, 0.5% yeast powder, 0.41‰ magnesium sulfate, the rest is water, pH7.0-7.2;

[0045] The fermented bacterial liquid obtained b...

Embodiment 2

[0051] The fermentation preparation of microbial bacterial liquid is basically the same as embodiment 1;

[0052] The parts by weight of each component of the compound nutrient are: 8 parts of urea, 4 parts of monoammonium phosphate, 4 parts of potassium sulfate, 80 parts of potassium dihydrogen phosphate, 15 parts of chelated calcium, 10 parts of magnesium sulfate, 3 parts of sodium silicate, Zinc sulfate 5 parts, manganese sulfate 2 parts, boric acid 8 parts, ferrous sulfate 5 parts, copper sulfate 2.5 parts, humic acid 0.5 parts, soybean peptone 8 parts, compound amino acid 2 parts, wheatgrass powder 3 parts.

[0053] The parts by weight of each component of the compound amino acid are: 5 parts of alanine, 10 parts of arginine, 5 parts of aspartic acid, 3 parts of cystine, 2 parts of glutamic acid, 2 parts of glycine, histamine 3 parts of acid, 10 parts of lysine, 3 parts of methionine, 5 parts of phenylalanine, 2 parts of proline, 1 part of serine, 10 parts of threonine, 5...

Embodiment 3

[0056] The fermentation preparation of microbial bacterial liquid is basically the same as embodiment 1;

[0057] The parts by weight of each component of the compound nutrient are: 88 parts of urea, 4 parts of monoammonium phosphate, 4 parts of potassium sulfate, 10 parts of chelated calcium, 6 parts of magnesium sulfate, 6 parts of chelated magnesium, 2 parts of sodium silicate, sulfuric acid 3 parts of zinc, 3 parts of chelated zinc, 2 parts of manganese sulfate, 2 parts of chelated manganese, 5 parts of boric acid, 5 parts of chelated iron, 2.5 parts of chelated copper, 0.5 parts of humic acid, 3 parts of soybean peptone, 3 parts of compound amino acids parts, 5 parts of wheatgrass powder.

[0058] The parts by weight of each component of the compound amino acid are: 5 parts of alanine, 10 parts of arginine, 5 parts of aspartic acid, 3 parts of cystine, 2 parts of glutamic acid, 2 parts of glycine, histamine 3 parts of acid, 10 parts of lysine, 3 parts of methionine, 5 pa...

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Abstract

The invention discloses a composite microbial fertilizer containing polyglutamate high-producing strain, a preparation method and an application thereof, belonging to a field of the composite microbial fertilizers. The method comprises preparing Bacillus licheniformis, Bacillus megaterium, Bacillus thuringiensis and Bacillus amyloliquefaciens into bacteria liquid through liquid fermentation, and mixing with complex composite nutrients according to a mass ratio of 1 : 1 to obtain the high-efficiency liquid-state composite microbial fertilizer. On basis of a good phosphate dissolving capability of the Bacillus megaterium, a good pest-resistant capability of the bacillus thuringiensis and a good organic matter degrading capability of the Bacillus amyloliquefaciens, the fertilizer combines performances of excellent water retention, fertilizer retention and non pollution to the environment of gamma-polyglutamate produced by the Bacillus licheniformis in a high yield, so as to achieve a relatively good fertilizer efficiency and relatively few using amount of water, and thus form scientific and reasonable composite fertilizer match.

Description

technical field [0001] The invention belongs to the field of compound microbial fertilizers, and in particular relates to a compound microbial fertilizer containing polyglutamic acid high-yielding bacteria and its preparation method and application. Background technique [0002] Among the top ten countermeasures for the protection of the ecological environment in my country, it is clearly advocated to promote "ecological agriculture", protect the ecological environment and farmland soil, promote the production of pollution-free and healthy food, and ensure that a sufficient amount of organic matter returns to the soil to promote the biological cycle in the ecosystem. To achieve the purpose of maintaining and enhancing soil fertility and biological activity. Microbial agents can prevent diseases and are non-toxic and harmless to humans and animals. They are the preferred means of production for the production of high-quality and pollution-free agricultural products. With the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 乔长晟王凡李雪张苗苗盖丽丰李政
Owner 天津北洋百川生物技术有限公司
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