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Quantum dot immunochromatography test strip for quickly detecting beta-amyloid protein and application

An immunochromatographic test strip and amyloid technology, applied in the field of immunodetection, can solve the problems of rapid fluorescence decay of organic fluorescent dyes, easy to be bleached, limited application, etc.

Inactive Publication Date: 2019-01-11
万东山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

③Strong photostability, under continuous light excitation, the fluorescence of organic fluorescent dyes decays rapidly and is easy to be bleached, which largely limits their application in bioluminescence labeling

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] (1) Preparation of nitrocellulose membrane with detection area and quality control area

[0071] ① Dilute Aβ40 monoclonal antibody and Aβ42 monoclonal antibody with 0.01M phosphate buffer containing 1% trehalose (pH=8, filter with 0.22μm before use) to a concentration of 1.5mg / mL, The DNP antibody was diluted to a concentration of 1 mg / mL to obtain the Aβ40 monoclonal antibody working solution, the Aβ42 monoclonal antibody working solution and the DNP antibody working solution;

[0072] ② Use the Aβ40 monoclonal antibody working solution, Aβ42 monoclonal antibody working solution and DNP antibody working solution prepared in step (1) to draw a film on the NC membrane with a film-drawing device, and the film-drawing speed is 1 μL / cm. Among them, Aβ40 The monoclonal antibody was drawn at 0.8cm, the Aβ42 monoclonal antibody was drawn at 1.3cm, and the DNP antibody was drawn at 1.8cm to form the detection area T1, detection area T2 and quality control area C, and then dried...

Embodiment 2

[0081] (1) Preparation of nitrocellulose membrane with detection area and quality control area

[0082] ① Dilute Aβ40 monoclonal antibody and Aβ42 monoclonal antibody with 0.01M phosphate buffer (pH=7.5, filter with 0.22μm before use) containing 1% trehalose to a concentration of 1.0mg / mL respectively, The DNP antibody was diluted to a concentration of 0.75mg / mL to obtain Aβ40 monoclonal antibody working solution, Aβ42 monoclonal antibody working solution and DNP antibody working solution;

[0083] ② Use the Aβ40 monoclonal antibody working solution, Aβ42 monoclonal antibody working solution and DNP antibody working solution prepared in step (1) to draw a film on the NC membrane with a film-drawing device, and the film-drawing speed is 1 μL / cm. Among them, Aβ40 The monoclonal antibody was drawn at 0.8 cm, the Aβ42 monoclonal antibody was drawn at 1.3 cm, and the DNP antibody was drawn at 1.8 cm to form the detection area T1, detection area T2 and quality control area C, and th...

Embodiment 3

[0092] (1) Preparation of nitrocellulose membrane with detection area and quality control area

[0093] ①Dilute Aβ40 monoclonal antibody and Aβ42 monoclonal antibody with 0.01M phosphate buffer (pH=8.5, filter with 0.22μm before use) containing 1% trehalose to a concentration of 2.5mg / mL respectively, DNP antibody was diluted to a concentration of 2.5mg / mL to obtain β40 monoclonal antibody working solution, Aβ42 monoclonal antibody working solution and DNP antibody working solution;

[0094] ② Use the Aβ40 monoclonal antibody working solution, Aβ42 monoclonal antibody working solution and DNP antibody working solution prepared in step (1) to draw a film on the NC membrane with a film-drawing device, and the film-drawing speed is 1 μL / cm. Among them, Aβ40 The monoclonal antibody was drawn at 0.8cm, the Aβ42 monoclonal antibody was drawn at 1.3cm, and the DNP antibody was drawn at 1.8cm to form the detection area T1, detection area T2 and quality control area C, and then dried a...

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Abstract

The invention belongs to the technical field of immunodetection and specifically relates to a quantum dot immunochromatography test strip for quickly detecting beta-amyloid protein and an application.The quantum dot immunochromatography test strip for quickly detecting beta-amyloid protein comprises nitrocellulose membranes arranged in a detection area and a quality control area, glass fiber membranes coating quantum dot micro-particles and coupled with Abeta40 and Abeta42 polyclonal antibodies, a base plate, a sample cushion and a water-absorbing cushion. The quantum dot immunochromatographytest strip for quickly detecting beta-amyloid protein provided by the invention is capable of realizing simultaneous detection of Abeta40 and Abeta42 antigen indexes through once reaction by utilizing a quantum dot immunochromatography technology, is capable of avoiding the problem of intersection of different proteins and has the advantages of short detection time, sensitivity 10 times or aboveof that of enzyme-linked immunosorbent method, high specificity, rapid response, high accuracy and quantification accuracy.

Description

technical field [0001] The invention belongs to the technical field of immunodetection, and in particular relates to a quantum dot immunochromatographic test strip for rapidly detecting β-amyloid protein and its application. Background technique [0002] Alzheimer's disease (Alzheimer disease, AD), first discovered by the Bavarian neuropathologist Alzheimer in 1907 and named after him, is a degenerative disease of the central nervous system with an insidious onset and a progressive course. Chronic and progressive, mainly manifested as neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes, and language barriers, which seriously affect social, occupational, and life functions. Alzheimer's disease is the most common type of senile dementia, accounting for about 50-60% of all dementias. According to statistics, in Western countries, the prevalence rate of the age group greater than or equal to 85 years is between 24-33%. Alt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558G01N33/577G01N33/543G01N33/58G01N33/532
CPCG01N33/532G01N33/54313G01N33/558G01N33/577G01N33/588G01N33/6896
Inventor 万东山郭兰英
Owner 万东山
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