Method for removing PVS viruses of potato test-tube plantlets
A technology for test-tube seedlings and potatoes, which is applied in the field of agricultural seedling cultivation, can solve problems such as poor treatment effects, achieve the effects of reducing detection costs, avoiding duplication of labor, and high detoxification rate
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[0015] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0016] A method for removing PVS virus of potato test-tube plantlets, which is characterized in that it comprises the following steps:
[0017] (1) Pretreatment of test tube seedling materials
[0018] The test tube seedlings containing PVS were tested to contain the top cut sections and placed in a culture flask containing 30-40mg / L ribavirin and 10-20mg / L butyryl hydrazide in MS solid medium, 15 plants per bottle, at a temperature of 23~ 25℃, light time 15h / d, light intensity 2000~3000lx, culture for 15~20d, remove the bottle seedlings contaminated by bacteria and fungi. When the longest root of the new seedling is 0.5cm...
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