Rapid propagation method of ginger virus-free seedlings by one step

Abstract

The invention discloses a rapid propagation method of ginger virus-free seedlings by one step. The rapid propagation method comprises the following steps: A, selecting tender shoots germinating on ginger rhizomes as explants; B, carrying out heat treatment to the explants, so as to detoxify the explants; C, disinfecting the detoxified explants with ethyl alcohol and mercury bichloride; D, peeling stem tips by a dissecting microscope; E, inducing the stem tips to grow micro ginger cluster buds under light condition and constant temperature condition; F, carrying out virus detection on the micro ginger cluster buds by an enzyme-linked immunosorbent assay (ELISA); G, carrying out subculture multiplication culture on the virus-free micro ginger cluster buds; H, transplanting virus-free micro ginger cluster buds with roots after plant division. By the utilization of the rapid propagation method, various viruses and germs in ginger bodies are removed effectively, germ-free and virus-free ginger seedlings are cultured, virus-free healthy cultivation of the ginger is realized, space occupation of propagation is small, propagation speed is high, one virus-free bud can propagate more than hundred thousand virus-free ginger seedlings for one year, a problem of virus-free ginger seedling supplying in plantation of ginger is solved effectively, good quality of the ginger is recovered, the disease occurrence is reduced, and the output and quality of the ginger are improved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for rapidly multiplying virus-free ginger seedlings in one step. The method utilizes a basic medium and adds different hormone components to achieve rapid propagation of virus-free ginger seedlings. Background technique [0002] Ginger (Zingiber officinale Rosc.) is a perennial herbaceous plant belonging to Zingiberaceae Zingiberaceae. Young ginger can be used as vegetables, and old ginger can be used as spices and medicines. It is an important medicinal and edible economic crop. Ginger likes a warm and humid climate. It is not resistant to cold, is afraid of humidity, and is afraid of direct sunlight. It is a good helper for farmers in mountainous areas to get rid of poverty and become rich. Because ginger does not bloom or rarely blooms, ginger reproduces asexually for a long time, resulting in the accumulation of various pathogenic bacteria and...

AI Technical Summary

Problems solved by technology

Ginger blast has become a restrictive factor for the development of ginger production. The diseased plots generally reduce production by 20-30%, and the severe cases reach 50-70%, or even no harvest; the accumulation of ginger virus usually leads to a 5-45% reduction in ginger production, while making it Reduced quality and reduced stress resistance

At the same time, in conventional cultivation, the germinated ginger is cut into pieces for planting, and 4500-6000 kg of ginger is needed per hectare, which not only consumes a large amount of commercial ginger, but also requires a large amount of land to reserve seeds, and the production cost is relatively high

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