Rapid propagation method of ginger virus-free seedlings by one step
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A technology of virus-free seedlings and ginger, which is applied in the field of plant tissue culture, can solve the problems of ginger yield decline, consumption of commercial ginger, and stress resistance decline, and achieve the effects of shortening the production cycle, reducing production costs, and shortening the cycle
Inactive Publication Date: 2013-10-16
湖北蔬谷农业科技有限公司 +1
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Problems solved by technology
Ginger blast has become a restrictive factor for the development of ginger production. The diseased plots generally reduce production by 20-30%, and the severe cases reach 50-70%, or even no harvest; the accumulation of ginger virus usually leads to a 5-45% reduction in ginger production, while making it Reduced quality and red...
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Embodiment 1
[0021] Ginger one-step becomes the rapid propagation method of virus-free seedling, and its steps are:
[0022] 1. Select bright yellow, shiny, full and healthy ginger with many buds and no scars, rinse it with tap water, bury it in the washed sand bed, and store it at 20°C or 21°C or 22°C or 24°C or 24°C Or germinate at 25°C. When the shoots grow to 1cm or 1.5cm or 2cm long, cut them with a blade, rinse with tap water to remove the sand;
[0023] 2. The buds are placed in an incubator and treated at 50°C for 5 minutes to complete detoxification;
[0024] 3. Soak in 75% alcohol solution by volume for 10s or 15s or 20s or 25s or 30s on the ultra-clean workbench, rinse with sterile water for 3 times, and then disinfect with 0.1% (weight ratio) mercury chloride solution for 10min or 11min or 12min, wash with sterile water 3 times or 4 times or 5 times;
[0025] 4. After draining the water, dissect the processed material under a dissecting microscope under aseptic conditions, c...
Embodiment 2
[0031] Ginger one-step becomes the rapid propagation method of virus-free seedling, and its steps are:
[0032] 1. Select bright yellow, shiny, plump and robust ginger with many buds and no scars, rinse it with tap water, bury it in a washed sand bed, and accelerate germination at 25°C. When the shoots grow to 1.5cm long, cut them with a blade, rinse with tap water to remove the sand;
[0033] 2. The buds are placed in an incubator and treated at 50°C for 5 minutes to complete detoxification;
[0034] 3. Soak in 75% alcohol solution by volume for 20 seconds on the ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 0.1% (by weight) mercury solution for 12 minutes, and rinse with sterile water for 5 times;
[0035] 4. After draining the water, dissect the processed material under a dissecting microscope under aseptic conditions, cut out the 0.2mm growth point of the meristem, and quickly inoculate it into the induction medium. The active ingredien...
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Abstract
The invention discloses a rapid propagation method of ginger virus-free seedlings by one step. The rapid propagation method comprises the following steps: A, selecting tender shoots germinating on ginger rhizomes as explants; B, carrying out heat treatment to the explants, so as to detoxify the explants; C, disinfecting the detoxified explants with ethyl alcohol and mercury bichloride; D, peeling stem tips by a dissecting microscope; E, inducing the stem tips to grow micro ginger cluster buds under light condition and constant temperature condition; F, carrying out virus detection on the micro ginger cluster buds by an enzyme-linked immunosorbent assay (ELISA); G, carrying out subculture multiplication culture on the virus-free micro ginger cluster buds; H, transplanting virus-free micro ginger cluster buds with roots after plant division. By the utilization of the rapid propagation method, various viruses and germs in ginger bodies are removed effectively, germ-free and virus-free ginger seedlings are cultured, virus-free healthy cultivation of the ginger is realized, space occupation of propagation is small, propagation speed is high, one virus-free bud can propagate more than hundred thousand virus-free ginger seedlings for one year, a problem of virus-free ginger seedling supplying in plantation of ginger is solved effectively, good quality of the ginger is recovered, the disease occurrence is reduced, and the output and quality of the ginger are improved.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for rapidly multiplying virus-free ginger seedlings in one step. The method utilizes a basic medium and adds different hormone components to achieve rapid propagation of virus-free ginger seedlings. Background technique [0002] Ginger (Zingiber officinale Rosc.) is a perennial herbaceous plant belonging to Zingiberaceae Zingiberaceae. Young ginger can be used as vegetables, and old ginger can be used as spices and medicines. It is an important medicinal and edible economic crop. Ginger likes a warm and humid climate. It is not resistant to cold, is afraid of humidity, and is afraid of direct sunlight. It is a good helper for farmers in mountainous areas to get rid of poverty and become rich. Because ginger does not bloom or rarely blooms, ginger reproduces asexually for a long time, resulting in the accumulation of various pathogenic bacteria and...
Claims
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