Rapid propagation method for Aletris stelliflora Hand.-Mzt
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A pollen and vegetable technology, applied in the field of plant tissue culture, can solve the problems of difficult germination, low seed setting rate, and large-scale planting without manual labor, and achieve the effects of low cost, short cycle, and small space occupied by reproduction
Inactive Publication Date: 2014-07-23
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI +1
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Problems solved by technology
Survive the winter with underground stems, sprout new shoots in spring, and flower stems are pumped from May to July, but the seed setting rate is low and it is not easy to germinate
Due to the limitation of seedlings, there is no relevant report on artificial large-scale planting
Method used
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Embodiment 1
[0019] A method for rapid propagation of star flower vermicelli noodles, its steps are:
[0020] 1. Take the healthy and plump seeds of Starflower Noodles, first soak in 1% mass ratio washing powder water for 4min or 5min or 6min, then rinse with tap water, use 75% (volume ratio) alcohol on the ultra-clean workbench Soak the surface of the solution for about 10s or 15s or 20s or 25s or 30s, rinse with sterile water 3 or 4 or 5 times, and then disinfect with 0.1% (weight ratio) mercury solution for 10min or 11min or 12min or 13min or 14min or 15min , Rinse 3 times or 4 times or 5 times with sterile water and inoculate them into MS medium.
[0021] 2. Light cultivation, temperature 25℃ or 26℃ or 27℃ or 28℃, light intensity 1500Lx or 2000Lx or 2500Lx or 3000Lx, light for 10h or 12h or 14h or 16h every day, cultivate for 30d or 40d or 50d or 60d, seed germination Seedlings with a height of 0.2cm or 0.3cm or 0.4cm or 0.5cm;
[0022] 3. Under aseptic conditions, use a sterilized scalpel ...
Embodiment 2
[0027] A method for rapid propagation of star flower vermicelli noodles, its steps are:
[0028] 1. Take the healthy and plump seeds of Starflower Noodles, soak them in 1% by weight washing powder water for 5 minutes, then rinse them with tap water, and soak them in a 75% (volume ratio) alcohol solution on a clean bench for 30s. , Rinse 5 times with sterile water, then sterilize with 0.1% (weight ratio) mercury solution for 15 min, rinse 5 times with sterile water, and inoculate it into MS medium.
[0029] 2. Light cultivation, the temperature is 25℃, the light intensity is 2000Lx, the light is 14h per day, cultivated for 50d, the seedlings will germinate with 0.3cm height;
[0030] 3. Under aseptic conditions, use a sterilized scalpel to cut the hypocotyls and inoculate them in the prepared clump bud induction medium. The formula of the induction medium is: MS+6-BA2.0mg / L+NAA0. 4mg / L+agar 5000mg / L+white sugar 30000mg / L;
[0031] 4. Light cultivation, the temperature is 25℃, the ligh...
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Abstract
The invention discloses a rapid propagation method for Aletris stelliflora Hand.-Mzt. The method comprises the steps as follows: A, selecting seeds of the Aletris stelliflora Hand.-Mzt as an explant; B, disinfecting the explant with ethyl alcohol and mercury bichloride and washing the explant with sterile water; C, inoculating the explant to an MS medium for germination; D, cutting a lower germinated plumular axis as an explant for induction of a clustered bud under the aseptic condition; E, cutting the clustered bud into a plurality of small clustered buds and conducting multiplication culture; G, transferring a robust seedling to a root medium in the process of subculture, opening a bottle to harden the seedling after the root is grown, cleaning and then transplanting the seedling to a medium, preserving heat and moisture, and transplanting after the tissue cultured seedling survives. The obtained Aletris stelliflora Hand.-Mzt is good in genetic stability, occupies small propagation space, and is rapid in propagation, and ten thousand of Aletris stelliflora Hand.-Mzt can be propagated from one seed in a year. The problem of supply of the excellent seedling of Aletris stelliflora Hand.-Mzt is effectively solved in the planting of the Aletris stelliflora Hand.-Mzt. The built production system is stable, has a relatively short period, and can meet the requirement of the seedling in the large-scale planting.
Description
Technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a rapid propagation method of the star flower vermicelli, which uses a basic culture medium and additional different components to rapidly propagate the star flower vermicelli. Background technique [0002] Aletris stelliflora Hand.-Mzt (Aletris stelliflora Hand.-Mzt) is a herbaceous plant belonging to the genus of Liliaceae. It is also called Niuwei. Because of its thick tender stem, the index finger is thick and the length is about one foot long. vegetables. The tender stems are rich in vitamins, protein, potassium, iron, calcium, phosphorus and other trace elements and dietary fiber, and have the functions of clearing heat, detoxifying, anti-inflammatory, promoting body fluid and quenching thirst. The root or whole plant can also be used for medicinal purposes. According to current data records, Xinghuaniantiaoercai is only found in alpine meadows, al...
Claims
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭凤领吴金平丁自立邓晓辉陈磊夫邱正明
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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