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Antibiotic detection method based on molecular imprinting-Raman spectrum

A technology of Raman spectroscopy and molecular imprinting, applied in Raman scattering, measuring devices, analysis materials, etc., can solve the problems affecting the efficiency of sample pretreatment and reduce the application value of environmental trace pollutant pretreatment, etc., and achieve short detection time , fast adsorption, good sensitivity

Active Publication Date: 2019-04-05
JIANGSU INST OF ECONOMIC & TRADE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, common solid-phase extraction methods lack selectivity. Therefore, while absorbing and concentrating trace pollutants in the environment, other high-concentration pollutants will also be extracted at the same time, which affects the efficiency of sample pretreatment and reduces its concentration in environmental traces. Application value in the pretreatment of pollutants

Method used

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  • Antibiotic detection method based on molecular imprinting-Raman spectrum
  • Antibiotic detection method based on molecular imprinting-Raman spectrum
  • Antibiotic detection method based on molecular imprinting-Raman spectrum

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Step 1, preparation of nano-gold sol: add 4 mL of 1% HAuCl to 120 mL of 4 °C deionized water 4 , stirred, and then added 1mL 0.2mol·L -1 K 2 CO 3 Solution, add 5mL 0.5mg·mL quickly at the end -1 NaBH 4 solution, stirred for 3 minutes, and the obtained wine red nano-gold sol (AuNPs) solution;

[0037] Step 2, PNM-Au composite hydrogel: add 1g N-isopropylacrylamide, 0.2g tetracycline, 15mg sodium hexadecylsulfonate, 15mg cross-linking agent in a flask with condensing reflux and magnetic stirring device A solution composed of N,N-methylene bisacrylamide, 120mL deionized water and 20mL nano-gold sol solution, dispersed evenly under the ultrasonic field, heated to 65°C under the protection of nitrogen flow, and quickly added 8mL ammonium persulfate solution prepared , pour the solution into the mold to react, after the reaction, a film-like gel is formed, after cutting the gel, use electric field dialysis to purify, remove surfactants, unreacted monomers and other subst...

Embodiment 2

[0042] Step 1, preparation of nano-gold sol: add 6 mL of 1% HAuCl to 180 mL of 4 °C deionized water 4 , stirred, and then added 3mL 0.2mol·L -1 K 2 CO 3 Solution, add 10mL 0.5mg·mL quickly at the end -1 NaBH 4 solution, stirred for 6min, and the obtained wine red nano-gold sol (AuNPs) solution;

[0043] Step 2, PNM-Au composite hydrogel: add 3g N-isopropylacrylamide, 0.4g tetracycline, 25mg sodium cetylsulfonate, 25mg cross-linking agent in a flask with condensing reflux and magnetic stirring device A solution composed of N,N-methylenebisacrylamide, 160mL deionized water and 30mL nano-gold sol solution, dispersed evenly under the ultrasonic field, heated to 75°C under the protection of nitrogen flow, and quickly added 12mL ammonium persulfate solution prepared , pour the solution into the mold to react, after the reaction, a film-like gel is formed, after cutting the gel, use electric field dialysis to purify, remove surfactants, unreacted monomers and other substances, a...

Embodiment 3

[0048] Step 1, preparation of nano-gold sol: add 5 mL of 1% HAuCl to 140 mL of 3 °C deionized water 4 , stirred, and then added 2mL 0.2mol·L -1 K 2 CO 3 solution, add 9mL 0.5mg·mL quickly at the end -1 NaBH 4 solution, stirred for 5min, and the obtained wine red nano-gold sol (AuNPs) solution;

[0049] Step 2, PNM-Au composite hydrogel: add 2g N-isopropylacrylamide, 0.3g tetracycline, 20mg sodium cetylsulfonate, 20mg cross-linking agent in a flask with condensing reflux and magnetic stirring device A solution composed of N,N-methylene bisacrylamide, 140mL deionized water and 25mL nano-gold sol solution, dispersed evenly under the ultrasonic field, heated to 70°C under the protection of nitrogen flow, and quickly added 10mL ammonium persulfate solution prepared , pour the solution into the mold to react, after the reaction, a film-like gel is formed, after cutting the gel, use electric field dialysis to purify, remove surfactants, unreacted monomers and other substances, a...

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Abstract

The invention relates to an antibiotic detection method based on a molecular imprinting-Raman spectrum, and belongs to the technical field of food detection. Antibiotics are used as template molecules; a composite nanometer material (MIP-PNM-Au) composed of an antibiotic molecular imprinted PNIPAM temperature-sensitive gel film and nanogold is provided; and a Raman enhancement effect of the composite material is utilized, so that the enrichment and the ultra-sensitive Raman spectrum detection of the antibiotics are realized. According to the method, a specific recognition function of a molecular imprinting technology (MIP), an intelligent response characteristic of gel and an SERS property of the nanogold are combined. The MIP-PNM-Au composite material is improved in selective enrichment effect through the MIP, and parameters such as the density, the interval and the like of nanoparticles are adjusted by means of the temperature-sensitive property of the gel, so that the surface plasmaresonance and the electromagnetic field intensity are tuned, dynamic generation of "hot spots" is realized, and the Raman enhancement effect is optimized.

Description

technical field [0001] The invention relates to an antibiotic detection method based on molecular imprinting-Raman spectroscopy, which belongs to the technical field of food detection. Background technique [0002] Antibiotics are a class of secondary metabolites produced by higher animals, plants or microorganisms during their metabolism [0003] It is a chemical substance that can interfere with the development and function of pathogenic bacteria. Self-resistance [0004] Since the discovery of vitamins, they have been widely used in humans, aquatic products and [0005] Bacterial disease control and treatment of livestock and poultry. However, since entering the 21st century, the worldwide abuse of antibiotics [0006] Serious, especially in my country. According to relevant surveys, the amount of antibiotics used in my country reached 162,000 tons in 2013, accounting for about half of the world's consumption, of which 48% were used for humans, 52% were used for liv...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 蒋彩云张沐熙毛宇欣崔逸
Owner JIANGSU INST OF ECONOMIC & TRADE TECH
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