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Antibiotic test paper strip and preparation method and application thereof

A technology for detecting test strips and antibiotics, applied in the field of immunological detection, can solve the problems of high cost and time-consuming, and achieve the effects of low production cost, convenient portability and rapid detection.

Inactive Publication Date: 2016-03-23
广州璞雅医药生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the time-consuming and high-cost defects of β-lactams, tetracyclines and sulfa antibiotics in the prior art, and provide a highly sensitive, convenient and quick β-lactams, tetracyclines and sulfa antibiotics. Sulfonamide antibiotic detection test strip and its preparation method and application

Method used

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  • Antibiotic test paper strip and preparation method and application thereof
  • Antibiotic test paper strip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of test strip

[0028] 1.1 Preparation of colloidal gold particles

[0029] Take 0.01% HAuCl 4 Put 100mL of aqueous solution in a beaker; add 2mL of 1% trisodium citrate aqueous solution, heat and boil with a constant temperature electromagnetic stirrer for about 15min, until the solution turns red; add 0.5mL0.2mol / LK after cooling at room temperature 2 CO 3 Mix well. The diameter of the colloidal gold particles prepared by this method is 15nm, and the size is uniform. The prepared colloidal gold particles were stored in a 4°C refrigerator for later use.

[0030] 1.2 Preparation of specific antibody-colloidal gold marker

[0031] Take 30mL colloidal gold solution and add it to a 50mL centrifuge tube, use 0.1MK 2 CO 3 Or 0.1M HCl to adjust the pH to 7.0-7.5, wrap the centrifuge tube with aluminum foil, and oscillate gently; add 1mL of β-lactam-specific antibody solution containing 100ug dropwise while oscillating, and continue to osci...

Embodiment 2

[0040] Embodiment 2: the application of test strip in milk detection antibiotic

[0041] 2.1 Sample addition

[0042] Take 200ug of fresh milk and drop it directly on the sample pad 2, or insert the sample pad 2 of the test strip into the fresh milk, but do not exceed the MAX mark line on the sample pad.

[0043] 2.2 Result Judgment

[0044] Start timing after adding the sample. Generally, it should be read at 5-10 minutes. The results at other times are invalid. It is preferable to read the results at 6 minutes of incubation.

[0045] 2.2.1 Judgment criteria for results

[0046] Such as figure 2 as shown,

[0047] Negative (-): When the antibiotic concentration in the sample is lower than the minimum detection concentration of the test strip, the color of the detection line B, T or S is darker or the same as that of the quality control line C, indicating that the residual amount does not exceed the standard, and it is judged as negative, such as figure 2 As shown, the ...

Embodiment 3

[0056] Embodiment 3: Sensitivity and specificity test of test strip

[0057] 3.1 Sensitivity detection

[0058] The β-lactam antibiotics and the sulfonamide antibiotics are respectively diluted to different concentrations with 0.2mol / L phosphate buffer solution with a pH of 7.4, then detected with the test strip of the present invention, and the antibiotics of different concentrations are added dropwise to Sample pad 2 for test strips. The results showed that the detection sensitivities of this test strip to β-lactam antibiotics, cephalosporin antibiotics and sulfonamide antibiotics were: penicillin G 4 μg / L, ampicillin 4 μg / L, amoxicillin 4 μg / L, oxacillin 30 μg / L, o-penicillin 30μg / L, dipenicillin 30μg / L, nafcillin 30μg / L, cefquinol 20μg / L, cephalosporin 125μg / L, cefuroning 20μg / L, cefazolin 50μg / L, cephalosporin Piperidone 50 μg / L, cefepime 60 μg / L, ceftiofur 100 μg / L, cephalexin 100 μg / L, sulfadoxine 100 μg / L, sulfadiazine 100 μg / L, sulfamethoxazole 100 μg / L, sulfadiol ...

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Abstract

The invention discloses an antibiotic test paper strip. The antibiotic test paper strip comprises a bottom plate where a sample pad, a combined pad, a reaction membrane and a water absorbing pad sequentially adhere to. A detection line B covered with beta-lactam antigens, a detection line T covered with tetracycline antigens, a detection line S covered with sulfonamide antigens and a quality control line C covered with a goat-anti-mouse IgG are arranged on the reaction membrane, and the combined pad is covered with beta-lactam specific antibody-colloidal gold markers, sulfonamide specific antibody-colloidal gold markers and tetracycline specific antibody-colloidal gold markers. The invention further discloses a preparation method of the test paper strip and application of the test paper strip to milk antibiotic detection. The test paper is fast in detection, convenient to carry, high in sensitivity, easy and convenient to operate, convenient to store, long in shelf life, capable of being industrially produced, low in production cost and capable of detecting beta-lactam antibiotics, tetracycline antibiotics and sulfonamide antibiotics at a time.

Description

technical field [0001] The invention relates to the field of immunological detection, in particular to a test strip for detecting β-lactams, tetracyclines and sulfonamide antibiotics, a preparation method and application thereof. Background technique [0002] Dairy cows are susceptible to diseases during the breeding process. Due to the high efficacy and low price of antibiotics, dairy farmers use a large amount of antibiotics when cows are sick, especially β-lactams, tetracyclines and yellow amine antibiotics are used in large quantities, resulting in raw milk Antibiotic residues are common in the human body. Long-term intake of antibiotic-containing foods can cause drug accumulation. When the concentration reaches a certain level, it will bring a series of negative effects on human health, such as allergic reactions and damage to the balance of gastrointestinal flora. and enhance bacterial resistance. More importantly, antibiotics are excessively ingested into the intesti...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 梁展洪杨漾
Owner 广州璞雅医药生物科技有限公司
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