Method for diagnosing soil contamination by tetracycline antibiotics

Abstract

The invention discloses a method for diagnosing soil contamination by tetracycline antibiotics. The method includes the following steps: placing plant seeds in a group of soil with the antibiotic concentration being in gradient distribution for cultivation, after three days of cultivation, detecting the length of the root systems of the plant seeds and calculating the average value of the root length; establishing a relation curve between the antibiotics concentration in the soil and the average value of the root length; performing pretreatment on the to-be-detected soil containing antibiotics, placing plant seeds in the to-be-detected soil for cultivation, after three days of cultivation, detecting the length of the root systems of the plant seeds, and calculating the concentration of antibiotics in the to-be-detected soil according to the relation curve, wherein the plant seeds are Zaoshu No.8, the soil is blue purple clay soil, antibiotics are oxytetracycline and chlortetracycline, and the gradient range of antibiotic concentration in the soil is 0-5 mg.kg<-1>. The method provided by the invention can reflect the concentration of antibiotics in the to-be-detected sample, and has the characteristics of being simple, fast and low in cost, and the limitations of the traditional antibiotics detection method are overcome.

Description

technical field [0001] The invention belongs to the technical field of remediation of polluted environment, and in particular relates to a method for diagnosing soil tetracycline antibiotic pollution. Background technique [0002] Antibiotics are widely used in the prevention and treatment of various diseases in humans, livestock and poultry industry and aquaculture. However, most antibiotics cannot be completely absorbed by the body, and are excreted into the environment in the form of prototype or metabolites through the feces and urine of patients, livestock and poultry, and pollute livestock food, poultry food, aquatic products, water bodies and soil through different channels, and then endanger human health through the food chain. [0003] China is one of the countries where the abuse of antibiotics is the most serious in the world. On the one hand, antibiotics can cause organ damage, and on the other hand, they can cause bacterial drug resistance to increase, forming ...

AI Technical Summary

Problems solved by technology

At present, the detection methods of antibiotic residues mainly include high performance liquid chromatography, chromatography / mass spectrometry, etc. Most of the detection methods have certain reliability and good sensitivity, but these methods also have certain limitations, such as Time-consuming, cumbersome operation, requires expensive instruments, and is also limited by the detection limit of the instrument; there are also some rapid detection methods, such as microbial inhibition method, enzyme-linked immunoassay, etc., these methods have relatively high requirements for experimental conditions, and the reagents are easy to use. contaminated; and these traditional detection methods can only quantitatively calculate the concentration of antibiotics, but cannot assess the biological toxicity of antibiotics

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