Method for extracting, enriching and quantifying trace pipemidic acid on suspended particulate matters in water

Abstract

The invention discloses a method for extracting, enriching and quantifying trace pipemidic acid on suspended particulate matters in water. The method comprises the following steps: filtering a target water sample by using a micro-pore fiber membrane; collecting the fiber membrane after filtration; airing the fiber membrane and cutting the fiber membrane into fragments; putting the fragments into a triangular flask; adding an extracting agent and sealing the triangular flask; oscillating and extracting by ultrasonic waves; filtering an extracting liquid by using an organic fiber membrane; meanwhile, transferring the filtered extracting liquid into a K-D concentration bottle; adding a dehydration drying agent into an extracted filtering liquid; after absorbing water, putting the K-D concentration bottle into a rotary evaporator and concentrating; blowing concentrated liquid by nitrogen gas until the volume is smaller than 1mL; making up the concentrated extracted filtering liquid to be 1mL and transferring the concentrated extracted filtering liquid to an Agilent special bottle; detecting the liquid by adopting an internal standard method through a high performance liquid chromatography cascaded three-grade gas chromatograph-mass spectrometer; and analyzing a chromatograph-mass spectrum analysis pattern to finish detection. According to the method, the content of pipemidic acid adsorbed on the suspended particulate matters in a water environment can be supplemented and the blank of the detection of antibiotics can be filled.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a detection method for pipemidic acid in a water environment, in particular to a quantitative method for extracting and enriching pipemidic acid on particles in water. Background technique [0002] Pipemidic acid is a second-generation quinolone antibiotic drug. Its antibacterial activity and antibacterial spectrum are stronger and wider than nalidixic acid (nalidixic acid), but not as good as fluoroquinolones. Bacillus, Klebsiella, and Shigella have good antibacterial effects. No cross-resistance with various antibiotics. Clinically, it is mainly used for acute or chronic pyelonephritis, urinary tract infection, cystitis, bacillary dysentery, otitis media, etc. caused by sensitive bacteria. [0003] Pipemidic acid can be partially absorbed after oral administration, most of which are not metabolized and excreted through the kidneys in the original form. 24 hours...

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Problems solved by technology

These methods are limited to the quantification of dissolved pipemidic acid

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