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Method for the extraction, enrichment and quantification of trace pipemidic acid on suspended particulate matter in water

A technology for suspending particulate matter and water suspension, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve problems such as limitations

Active Publication Date: 2016-01-06
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are limited to the quantification of dissolved pipemidic acid

Method used

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  • Method for the extraction, enrichment and quantification of trace pipemidic acid on suspended particulate matter in water
  • Method for the extraction, enrichment and quantification of trace pipemidic acid on suspended particulate matter in water
  • Method for the extraction, enrichment and quantification of trace pipemidic acid on suspended particulate matter in water

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Embodiment Construction

[0035] The technical solution of the present invention will be further described below, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the protection scope of the present invention .

[0036] Such as figure 1 As shown, the method for extracting, enriching and quantifying trace amounts of pipemidic acid on suspended particles in water provided by the invention, its specific implementation steps are as follows:

[0037] (1) Take 2000ml of the target water sample, filter it with a 0.45μm microporous fiber membrane, collect the filtered filter membrane, dry the filter membrane, cut it into pieces and put it in the conical flask, measure an appropriate amount of methanol with a measuring cylinder and pour it into the conical flask , the volume ratio of the mass of the suspended particulate...

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Abstract

The invention discloses a method for extracting, enriching and quantifying trace pipemidic acid on suspended particulate matters in water. The method comprises the following steps: filtering a target water sample by using a micro-pore fiber membrane; collecting the fiber membrane after filtration; airing the fiber membrane and cutting the fiber membrane into fragments; putting the fragments into a triangular flask; adding an extracting agent and sealing the triangular flask; oscillating and extracting by ultrasonic waves; filtering an extracting liquid by using an organic fiber membrane; meanwhile, transferring the filtered extracting liquid into a K-D concentration bottle; adding a dehydration drying agent into an extracted filtering liquid; after absorbing water, putting the K-D concentration bottle into a rotary evaporator and concentrating; blowing concentrated liquid by nitrogen gas until the volume is smaller than 1mL; making up the concentrated extracted filtering liquid to be 1mL and transferring the concentrated extracted filtering liquid to an Agilent special bottle; detecting the liquid by adopting an internal standard method through a high performance liquid chromatography cascaded three-grade gas chromatograph-mass spectrometer; and analyzing a chromatograph-mass spectrum analysis pattern to finish detection. According to the method, the content of pipemidic acid adsorbed on the suspended particulate matters in a water environment can be supplemented and the blank of the detection of antibiotics can be filled.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a detection method for pipemidic acid in a water environment, in particular to a quantitative method for extracting and enriching pipemidic acid on particles in water. Background technique [0002] Pipemidic acid is a second-generation quinolone antibiotic drug. Its antibacterial activity and antibacterial spectrum are stronger and wider than nalidixic acid (nalidixic acid), but not as good as fluoroquinolones. Bacillus, Klebsiella, and Shigella have good antibacterial effects. No cross-resistance with various antibiotics. Clinically, it is mainly used for acute or chronic pyelonephritis, urinary tract infection, cystitis, bacillary dysentery, otitis media, etc. caused by sensitive bacteria. [0003] Pipemidic acid can be partially absorbed after oral administration, most of which are not metabolized and excreted through the kidneys in the original form. 24 hours...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 徐勇鹏崔福义时文歆田家宇沈吉敏齐宏赵晟锌
Owner HARBIN INST OF TECH
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