Method for fast, efficiently and synchronously detecting content of 11 kinds of antibiotics in soil and sludge

Abstract

The invention provides a method for simultaneously detecting 11 kinds of antibiotics in soil. The method utilizes an UPLC-MS / MS method to detect antibiotics and utilizes a standard addition method to determine a recovery rate of the detection method. The method mainly comprises extraction of antibiotics in soil, extraction and enrichment of the antibiotics and detection of the antibiotics. The method comprises (1) sample collection and preservation: collecting surface soil with thickness of 0-20cm, keeping a low temperature through an ice bag, carrying out dark preservation, cool-drying the collected soil sample, grinding the soil sample, sieving the soil sample through a sieve of 40 meshes, weighing 1g of the soil and carrying out antibiotic extraction, (2) extracting and purifying the extracted antibiotics in a form of SAX-HLB small column series connection, eluting the extract through 10ml of methanol, blowing nitrogen until complete drying, carrying out enrichment, carrying out volume metering through the pure methanol until the volume is 1 ml and feeding the solution into a small bottle, and (3) UPLC / MS / MS detection. The extraction solution used by the method comprises acetonitrile and a phosphate buffer solution with pH of 3 according to a ratio of 7: 3 and has the volume of 10 ml, the extraction process is carried out three times, the supernatant is merged and is subjected to SAX and HLB small column solid phase extraction, high purity nitrogen is blown to completely dry the extract, methanol is used until the solution volume is 1ml, and the solution is detected through the UPLC-MS / MS method.

Description

Technical field [0001] The invention belongs to the technical field of detection of environmental pollutants, and specifically relates to a rapid and efficient detection of chloramphenicol, florfenicol, furazolidone, enrofloxacin, ofloxacin, oxytetracycline and erythromycin in soil and sludge Sulfadiazine, sulfamethoxazole, ampicillin and tetracycline 11 types of antibiotics. Background technique [0002] Because antibiotics have a good antibacterial effect, they are widely used in the treatment of human and animal diseases, especially in the animal husbandry and aquaculture industries. This makes the detection in the environmental matrix and food at home and abroad different Concentration of antibiotics. The content of antibiotics in the environment not only severely disturbs the soil microbial gene pool and affects the safety of the ecosystem, but can also be enriched in the body of advanced animals through the food chain, and endanger the health of humans and animals. [0003]...

AI Technical Summary

Problems solved by technology

However, there are many kinds of antibiotics and metabolites in soil and sludge with low concentration, and various organic or inorganic impurities in soil and sludge will interfere with the detection of antibiotics

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