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Surface Enhanced Raman Spectroscopy (SERS) detection method for chlortetracycline hydrochloride

A technique of chlortetracycline hydrochloride and its detection method, which is applied in the field of SERS detection of chlortetracycline hydrochloride, can solve the problems of optimization, high detection limit, deviation of detection results, etc.

Active Publication Date: 2019-03-29
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are few reports on the detection of tetracycline antibiotics by surface-enhanced Raman scattering. Among them, Zhao et al. : 1-6.) Utilize SERS to detect tetracycline in duck meat, but the detection limit of the method used is higher (1.12mg / L), can not meet the requirement of trace detection
Chao et al. (K L Chao.A Simple Surface-Enhanced Raman Spectroscopic Method foron-Site Screening of Tetracycline Residue in Whole Milk[J].sensors,2018,18:424.) used SERS to detect tetracycline in milk and water, and its correlation The coefficients are 0.88 and 0.92 respectively, realizing on-site detection, but there is a large deviation in the detection results, which needs to be optimized

Method used

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  • Surface Enhanced Raman Spectroscopy (SERS) detection method for chlortetracycline hydrochloride
  • Surface Enhanced Raman Spectroscopy (SERS) detection method for chlortetracycline hydrochloride
  • Surface Enhanced Raman Spectroscopy (SERS) detection method for chlortetracycline hydrochloride

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Experimental program
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Effect test

Embodiment 1

[0032] 1) Synthesis of Au nanoparticles:

[0033] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle sol with a particle size of about 55 nm (see figure 1 ).

[0034] 2) Customized quartz sample holder:

[0035] Customized quartz sample holder with a diameter of 0.4cm and a thickness of 0.2cm (see figure 2 ).

[0036] figure 1 Scanning electron microscope image of Au nanoparticles prepared for step 1). exist figure 1 , the scale bar is 0.2μm ( figure 1 Figure A in ) and 20nm ( figure 1 in panel B). from figure 1 It can be seen that the Au nanoparticles are relatively uniform in size and approximately spherical.

[0037] figure 2 It is the quartz sampl...

Embodiment 2

[0039] 1) Synthesis of Au nanoparticles:

[0040] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle sol with a particle size of about 55 nm (see figure 1 ).

[0041] 2) Effects of different substrates on surface-enhanced Raman detection of aureomycin hydrochloride:

[0042] Get the solid powder of aureomycin hydrochloride, place it on a clean glass slide, cover with a cover glass, flatten the sample, and use a Witec Raman spectrometer to measure the conventional value of the solid powder of aureomycin hydrochloride at an excitation wavelength of 633nm. Raman spectrum. Before each test, use a silicon wafer at 520.6cm -1 Calibrate the instrument at the peak at t...

Embodiment 3

[0046] 1) Synthesis of Au nanoparticles:

[0047] Au nanoparticles (Au NPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle sol with a particle size of about 55 nm (see figure 1 ).

[0048] 2) Effects of different pH values ​​on the UV absorption spectrum and surface-enhanced Raman detection of chlortetracycline hydrochloride:

[0049] HCl and NaOH were used to adjust the pH values ​​of 0.1 mol / L aureomycin hydrochloride aqueous solution to 2, 6, and 13 respectively, and UV-visible spectrometer was used to detect the ultraviolet absorption of aureomycin hydrochloride aqueous solution under different pH conditions.

[0050] Take 1mL of the Au nanoparticles synthesized in step 1), conce...

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Abstract

The invention relates to a Surface Enhanced Raman Spectroscopy (SERS) detection method for chlortetracycline hydrochloride and relates to detection of tetracycline antibiotics. Au nanoparticle sol isprepared by reducing chloroauric acid with sodium citrate, and gold nanoparticles with uniform particle size are prepared by controlling the concentration of reactants, the reaction time, the reactiontemperature and the stirring speed; a SERS substrate is prepared with gold sol under constant temperature conditions. the pH of a chlortetracycline hydrochloride solution is controlled, fluorescent quencher is added, surface enhanced Raman detection is carried out on chlortetracycline hydrochloride on the SERS substrate, with the increase of the concentration of chlortetracycline hydrochloride, aRaman peak of chlortetracycline hydrochloride at a specific wavelength is gradually enhanced, and the intensity of the Raman characteristic peak of chlortetracycline hydrochloride is proportional tothe amount of chlortetracycline hydrochloride so as to carry out quantitative analysis and detection on chlortetracycline hydrochloride. The method has the advantages of low detection limit, high sensitivity, good reproducibility of detection, fast detection speed, high standard recovery rate of samples and the like and can meet the requirement for rapid analysis and detection.

Description

technical field [0001] The invention relates to the detection of tetracycline antibiotics, in particular to a SERS detection method of aureomycin hydrochloride. Background technique [0002] Tetracycline antibiotics can bind to the binding site of bacterial ribosomal 30s subunit RNA transcription, inhibit protein synthesis, and play a bactericidal role (AP Carter.Functional insights from the structure of the 30Sribosomal subunit and its interactions with antibiotics[J]. Nature, 2000, 407:340–348.). This type of antibiotic has good antibacterial activity and low cost of use. It is a class of broad-spectrum antibiotics commonly used in livestock, poultry and aquaculture to treat animal diseases, prevent and promote growth (F Granados-Chinchilla. Tetracyclines in food and feedingstuffs: From regulation to analytical methods, bacterial resistance, and environmental and health implications [J]. Journal of Analytical Methods in Chemistry. 2017, (1): 1-24.). Among them, chlortetr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 张芹贺路影苏文金
Owner JIMEI UNIV
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