Super-hydrophobic micro-pit array chip and preparation method and application thereof

A micro-pit array, super-hydrophobic technology, applied in biochemical equipment and methods, biochemical instruments, urinary tract/kidney cells, etc. Good compatibility, avoid cross-contamination effect

Active Publication Date: 2016-08-17
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, there is currently no platform that can be effectively used for high-throughput and high-resolution imaging. In the past, high-resolution imaging of multiple samples was performed by processing samples one by one, which was time-consuming, laborious, and inefficient, which largely lim

Method used

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  • Super-hydrophobic micro-pit array chip and preparation method and application thereof
  • Super-hydrophobic micro-pit array chip and preparation method and application thereof
  • Super-hydrophobic micro-pit array chip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1, preparation of superhydrophobic micropit array chip by micrografting

[0083] Schematic diagram of the structure of the superhydrophobic micropit array chip. figure 1 As shown, it includes a micro-pit array layer 1 and a super-hydrophobic layer 2 bonded on the surface of the micro-pit array layer, wherein the thickness of the super-hydrophobic layer 2 is 100 microns.

[0084] Such as figure 1 As shown, the superhydrophobic micropit array chip is prepared by micrografting technology, and the specific steps are as follows:

[0085] (a) Uniform glue: Taking Dow Corning 3140 as an example, a very thin layer of glue can be obtained on the PMMA layer at a speed of 7000rpm for 30s.

[0086] (b) PDMS micropit chip preparation: use the processed silicon wafer SU-8 mold (customized and processed by Boao Biochip Company), wrap it in tin foil and use it as an inverted mold container for later use. Preparation of PDMS prepolymer solution, the volume ratio of monomer an...

Embodiment 2

[0093] Example 2. Preparation of superhydrophobic micropit array chip by in situ synthesis

[0094] Schematic diagram (side view) of the structure of the superhydrophobic micropit array chip. Figure 5 As shown, it includes a base layer 1 and a superhydrophobic microporous array layer 2 attached to the surface of the substrate (the microporous array layer 2 is made of a superhydrophobic material).

[0095] Such as Image 6 As shown, the superhydrophobic micropit array chip was prepared by in situ synthesis technology, and the specific steps were as follows:

[0096] (a) Preparation of negative gel microcolumn array: uniformly 100 μm thick negative gel was applied on the silanized glass slide, exposed and developed to obtain a microcolumn array with a height of 100 μm, a diameter of 500 μm, and a center distance of 1000 μm.

[0097] (b) In-situ polymerization of the superhydrophobic layer: Align the glass slide with the micropit array with another silanized glass slide and cl...

Embodiment 3

[0099] Example 3. Superhydrophobic micropit array chip for ultra-high resolution imaging

[0100] This embodiment is based on the unique requirements of super-resolution imaging, and the preparation method of the micro-pit substrate array has been adjusted accordingly. The structure diagram of the super-hydrophobic micro-pit array chip and figure 1 Same, including the micropit array layer 1 and the superhydrophobic layer 2 bonded on the surface of the micropit array layer, only the micropit array layer 1 is composed of a base layer (special optical glass slide for ultra-high resolution imaging) and bonded on the substrate Composition of the microwell array layer on it.

[0101] Production process such as Figure 8 As shown, the specific steps are as follows:

[0102] (a) Uniform glue: Taking Dow Corning 3140 as an example, a very thin layer of glue can be obtained on the PMMA layer at a speed of 7000rpm for 30s.

[0103] (b) Preparation of photoresist dry film microarray: u...

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Abstract

The invention discloses a super-hydrophobic micro-pit array chip and a preparation method and application thereof. The super-hydrophobic micro-pit array chip comprises a micro-pit array layer, and the surface, except micro-pits or the bottoms of the micro-pits, of the micro-pit array layer is the super-hydrophobic surface. The super-hydrophobic micro-pit array chip can be characterized in that the surface except the micro-pits is the super-hydrophobic surface, and the super-hydrophobic micro-pit array chip comprises the micro-pit array layer and a super-hydrophobic layer attached to the surface of the micro-pit array layer; the surface except the bottoms of the micro-pits is the super-hydrophobic surface, the micro-pit array layer comprises a base layer and a micropore array layer attached to the surface of the base, and micropore array layer is made of a super-hydrophobic material. The super-hydrophobic micro-pit array chip can achieve complete isolation among cell microarrays at a low cost in the cell microarray construction process, cross contamination of the cell arrays is avoided, good biocompatibility is guaranteed, and the super-hydrophobic micro-pit array chip has no obvious influence on normal growth of various cells and is suitable for high-throughput cell detection and analysis.

Description

technical field [0001] The invention relates to a super-hydrophobic micropit array chip, a preparation method and application thereof. Background technique [0002] Cell experiments are one of the most basic research methods in biological research. Many biological studies, such as high-throughput drug screening, combined stem cell screening, etc., involve a large number of cytological experiments, through a large number of cell processing processes that are often very similar to optimize certain conditions or results; traditional experimental operations often These cell experiments are done manually in multi-well plates, which is time-consuming and labor-intensive, consumes a lot of reagents, and research costs are high. [0003] Cell microarray is a tiny cell array formed by chemical and physical separation. Such cell microarrays have attracted more and more attention in biological research because of their high throughput, low reagent consumption, and low cost. Chemical...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/071C12N5/09
CPCC12M23/12C12M23/20C12N5/0686C12N5/069C12N5/0694C12M3/00
Inventor 刘鹏张鹏飞张健雄边升太程一淳
Owner TSINGHUA UNIV
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