Molecular markers INHBA and SPP1 and application thereof

A technology of molecular markers and markers, applied in the direction of recombinant DNA technology, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem of inability to better meet clinical needs, incomplete clarity of molecular markers for lymph node metastasis, etc. problem, to achieve high accuracy, high repeatability, and small sample size

Pending Publication Date: 2019-10-01
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular markers related to lymph node metastasis are...

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  • Molecular markers INHBA and SPP1 and application thereof
  • Molecular markers INHBA and SPP1 and application thereof
  • Molecular markers INHBA and SPP1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Primary Screening of Example 1 Molecular Markers

[0034] First of all, establish a database of patients with gastric cancer that collects patient clinicopathological data, multi-level biological samples and follow-up data. Sexual (negative) lymph node samples.

[0035] After preliminary case screening: patients with gastric adenocarcinoma diagnosed by pathology, with detailed medical records and complete postoperative pathological data and follow-up data were included; neoadjuvant therapy was excluded; patients who underwent non-tumor resection such as laparotomy and gastrojejunostomy were excluded; Patients with other gastric malignancies and postoperative recurrence of gastric cancer were excluded; patients with simultaneous or metachronous combination of other primary malignancies were excluded.

[0036] Finally, 66 pairs of gastric primary tumor tissues and paired normal tissue samples, 50 cases of metastatic (positive) lymph node samples and 54 cases of non-metas...

Embodiment 2

[0038] Example 2 Detection of Expression Levels of Molecular Targets

[0039] Test samples: A total of 66 pairs of gastric primary tumor tissues and paired normal tissue samples, 50 cases of metastatic (positive) lymph node samples and 54 cases of non-metastatic (negative) lymph node samples collected were analyzed. All specimens were collected within half an hour of the tumor in vitro during gastric cancer resection. The tumor tissue was taken from the solid area of ​​the tumor to avoid the necrotic area, and the normal tissue was the gastric mucosa more than 4cm away from the tumor. All were cut into 0.5cm×0.5cm ×0.5cm in size and retained; perigastric lymph nodes refer to regional lymph nodes (lymph nodes 1 and 2), and enlarged perigastric lymph nodes with hard mass and diameter > 1 cm and soft non-swelled lymph nodes with diameter 1.8 and A260 / 230 close to 2.0 met the requirements.

[0040] Detection method: Detect the expression of the nine molecular targets in Example ...

Embodiment 3

[0051] Example 3 Kit

[0052] An embodiment of the early gastric cancer diagnostic kit of the present invention, which contains a primer pair for detecting the mRNA of INHBA and SPP1, wherein the base sequences of the primer pair are as shown in SEQ ID NO.7-8 and SEQ ID NO.9-10 Show.

[0053] An embodiment of the kit for predicting lymph node metastasis status of patients with gastric cancer of the present invention, which contains a pair of primers for detecting the mRNA of INHBA and SPP1, wherein the base sequences of the primer pairs are as SEQ ID NO.7-8 and SEQ ID NO. 9 to 10.

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Abstract

The invention discloses a set of markers used for early gastric cancer diagnosis. The markers are composed of INHBA and SPP1. The invention also discloses application of the INHBA and the SPP1 in preparing a kit for early gastric cancer diagnosis and a kit for predicting lymphatic metastasis status of a gastric cancer patient. The expression quantities of the INHBA and SPP1 are detected by adopting a semi-quantitative fluorescence real-time enzyme-linked reaction method, and compared with traditional pathological cytology detection, the detection of molecular markers has the advantages of small required sample size, specificity, sensitivity, accuracy, high repeatability, automation and the like. The molecular markers lay an ideal clinical application basis for the early gastric cancer diagnosis and the prediction of the lymphatic metastasis.

Description

technical field [0001] The invention relates to the technical field of gastric cancer detection, in particular to molecular markers INHBA and SPP1 and applications thereof. Background technique [0002] Gastric cancer is one of the most common malignant tumors in China. Gastric cancer in my country has the characteristics of high morbidity and mortality, low proportion of early gastric cancer, and advanced and metastatic gastric cancer cases. In recent years, with the rapid development of molecular biology technology, the application of molecular markers for tumor screening, diagnosis and prognosis has attracted more and more attention. However, there is no effective molecular marker with high sensitivity and specificity. features. Introducing a non-invasive or minimally invasive, objective, efficient, specific and sensitive new detection method for asymptomatic and high-risk groups is crucial to improving the diagnostic rate of early gastric cancer. At the same time, it ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 王玮方成周志伟
Owner SUN YAT SEN UNIV
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