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Antibody chip kit for detecting early gastric cancer

An antibody chip, an early detection technology, applied in biological testing, measurement devices, material inspection products, etc., can solve the problems of low sensitivity, single detection index, complicated operation, etc., and achieve low production cost, increased throughput, and time saving. Effect

Active Publication Date: 2016-05-11
RAYBIOTECH INC GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a high-throughput, high-sensitivity, high-specificity and low-cost early gastric cancer detection kit, specifically adopting antibody chip technology, which can detect five gastric cancer tumor markers at the same time, overcoming the existing technology It has defects such as cumbersome operation, single detection index, and low sensitivity. It has the advantages of cheap, convenient, sensitive, accurate, high-throughput, less sample consumption, and can be popularized and scaled in ordinary laboratories.

Method used

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  • Antibody chip kit for detecting early gastric cancer
  • Antibody chip kit for detecting early gastric cancer
  • Antibody chip kit for detecting early gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of antibody chip kit for detection of early gastric cancer.

[0041] 1. Components of the kit

[0042] 1. Solid-phase carrier: the reaction tank is formed by glass slides, soft silica gel pads, hard frames, and U-shaped frame clips. The hard frame is divided into 2X8 or 4X16 small holes to form a 16- or 64-hole frame (see figure 1 ). Wherein the size of the soft silicone pad corresponds to the hard frame and the standard glass slide. Each small grid of the rigid frame forms a small reaction tank, and the standard glass slides in each small reaction tank are combined with specific antibodies of corresponding concentrations.

[0043] 2. Washing solution: 20X concentrated washing solution containing Tween 20. 1X washing solution is pH 7.2, containing 0.1% Tween 20, 0.1mol / L phosphate buffer.

[0044] 3. Diluent: 2 bottles of 15ml 5X concentrated diluent D for diluting samples, 1 bottle of 15ml 5X concentrated diluent B for diluting antibody and HRP-strepta...

Embodiment 2

[0072] Prepare the kit according to the method of Example 1 with preferred antibodies specific to the five proteins of CEA, CA199, PGI, PGII, and CA724. Antibodies specific to 5 proteins according to Figure 4 Spot as shown. This kit was used to detect 8 clinical samples (the detection value of the clinical samples was known and measured by electrochemiluminescence method), and the detection method was the same as that described in Example 1. The detection value of the clinical sample is compared with the detection value of this kit and drawn as a scatter diagram, and the correlation is as follows: the detection result of electrochemiluminescence is basically equivalent to the detection result of this kit. The results are shown in Table 1 and Figure 5~9 .

[0073] Table 1

[0074]

[0075] Figure 5 The correlation between the CEA antibody of this kit and the detection results of the electrochemiluminescence kit is as follows, the trend equation is y=83.4x-125.1, and ...

Embodiment 3 Embodiment 2

[0076]The quality detection of the kit described in embodiment 3 embodiment 2

[0077] One, the detection sensitivity of each antibody of kit described in embodiment 2

[0078] The specific operation refers to Example 2, and the minimum detection limit and linear range of the kit are obtained by serially diluting each antigen. The test results are shown in Table 2.

[0079] Table 2

[0080]

[0081] Two, the cross-reaction experiment of each antibody of kit described in embodiment 2

[0082] The specific operation refers to Example 1, and the detection antibodies are anti-CEA antibody, anti-PGI antibody, anti-PGII antibody, anti-CA724 antibody, anti-CA199 antibody and the mixture of these five antibodies. The results of the cross-reactivity test of the antibody pair are as follows Figure 10 . Depend on Figure 10 The results show that each antibody pair can specifically recognize its own detection antigen without cross-reaction with other antigens.

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Abstract

The invention belongs to the technical field of chip detection and particularly discloses an antibody chip kit for detecting early gastric cancer. The antibody chip kit is characterized in that various gastric cancer marker related antibodies are fixed onto a solid-phase carrier, and the specific antibodies are screened from 35 routine gastric cancer marker related antibodies. The antibody chip kit can detect a plurality of gastric cancer related antigen proteins at the same time, and the detection effect of a combined antibody chip is better than that of single tumor markers.

Description

technical field [0001] The invention relates to the technical field of chip detection, in particular to an antibody chip kit for detecting early gastric cancer. Background technique [0002] my country is a high-incidence area of ​​gastric cancer, with an incidence rate as high as 40 / 100,000, second only to lung cancer, and has leapt to the second place in China's cancer incidence rate. In China, there are 400,000 new gastric cancer patients each year; accounting for 42% of the world's incidence; the prevalence and mortality are more than twice the world's average level; gastric cancer among young people aged 19 to 35 in the past five years The incidence rate has doubled compared with 30 years ago. Worldwide, gastric cancer is also one of the most common cancers with a high mortality rate. Therefore, the diagnosis and treatment of gastric cancer is a major project in the medical field. At present, in the comprehensive treatment of gastric cancer, surgery plays a dominant ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68G01N33/552G01N21/64
Inventor 黄若磐毛应清张卓
Owner RAYBIOTECH INC GUANGZHOU
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