Gene marker for screening early gastric cancer and application thereof

A gene marker and gastric cancer technology, applied in the field of molecular biology, can solve the problems of large manpower consumption, material resources, national burden, poor patient acceptance, etc., and achieve the effect of promoting early detection, broad application prospects, and improving the cure rate

Active Publication Date: 2017-01-11
SHANGHAI BIOMEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prognosis of gastric cancer is closely related to the timing of diagnosis and treatment. The 5-year survival rate of advanced gastric cancer is less than 30%, and the quality of life of patients is low, which brings a heavy burden to the family and the country.
There are many reasons for the low diagnosis and treatment rate of gastric cancer in my country. One of the important reasons is the lack of simple and effective screening methods for gastric cancer that can be used for general population census.
Endoscopy and endoscopic biopsy are currently the gold standard for the diagnosis of gastric cancer. However, endoscopic examination relies on equipment and endoscopist resources, and the cost of endoscopic examination is relatively high. It requires a lot of manpower and material resources for gastric cancer screening.
More importantly, diagnostic methods such as endoscopy are invasive examination methods, the examination process is painful, and the acceptance level of patients is poor, especially for many asymptomatic patients with low risk of gastric cancer.
Therefore, even for developed countries such as Japan, large-scale gastric cancer screening by endoscopy has not yet been adopted

Method used

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  • Gene marker for screening early gastric cancer and application thereof
  • Gene marker for screening early gastric cancer and application thereof
  • Gene marker for screening early gastric cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of Gastric Cancer Characteristic Gene Markers

[0024] The screening of gastric cancer characteristic genes includes the following steps:

[0025] 1) Using PAXgene TM Blood RNA Tube collects peripheral blood samples from 20 patients with gastric cancer confirmed by pathological examination, 20 healthy people, and 40 patients with five kinds of malignant tumors except gastric cancer. Each sample collects 2-3mL peripheral blood.

[0026] 2) The total RNA of the above peripheral blood samples was extracted using the PAXgene Blood RNA Kit extraction kit. Agilent Bioanalyzer 2100 bioanalyzer was used to detect the fragment integrity (RIN) of RNA samples, and the purity of RNA samples was detected by Nano1000 micro-ultraviolet spectrophotometer. All RNA samples must meet the following quality control conditions: RNA yield greater than 2 micrograms, 28S / 18S peak ratio greater than 1, RIN value greater than 7, and 260nm / 280nm absorbance ratio greater than...

Embodiment 2

[0035] Example 2 Using the screened gene markers of early gastric cancer to detect whether the subject suffers from gastric cancer

[0036] 1. Method steps:

[0037] 1) Collection of peripheral blood samples of samples to be tested: using PAXgene TM Blood RNA Tube collects 2-3ml peripheral blood samples from patients.

[0038] 2) Extraction and purification of total RNA in the peripheral blood samples of the samples to be tested: use the PAXgene Blood RNA Kit extraction kit to extract and purify the total RNA in the peripheral blood, and use the Agilent BioAnalyzer 2100 microelectrophoresis analyzer to identify the integrity of the extracted total RNA fragments The purity of the RNA sample was tested with a Nano1000 micro-volume ultraviolet spectrophotometer.

[0039] 3) Reverse transcription reaction: use the High-Capacity cDNA Reverse Transcription Kit reverse transcription kit from Life Techonolgy Company, use total RNA as template, and use Olig(dT) as reverse transcript...

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Abstract

The invention discloses a gene marker for screening early gastric cancer. The gene marker comprises a peripheral blood gastric cancer characteristic gene sequence shown as SEQ ID NO.1-SEQ ID NO.6. The invention also discloses application of the gene marker in preparation of products for screening early gastric cancer. The gene marker disclosed by the invention is used for screening early gastric cancer and is high in sensitivity and specificity; and moreover, because the peripheral blood which can be easily collected in clinical application is taken as a detection sample, the detection is convenient, the gene marker is applicable to screening and early diagnosis of a large population for gastric cancer, and the application prospects are wide.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a gene marker for early screening of gastric cancer and its application. Background technique [0002] Gastric cancer is a type of malignant tumor originating from the gastric mucosal epithelium. my country is a country with a high incidence of gastric cancer. Every year, there are about 400,000 new cases of gastric cancer and about 350,000 deaths. The number of deaths due to gastric cancer ranks third among all malignant tumor deaths. How to reduce the morbidity and mortality of gastric cancer in my country is a major public health problem to be solved urgently. The prognosis of gastric cancer is closely related to the timing of diagnosis and treatment. The 5-year survival rate of advanced gastric cancer is less than 30%, and the quality of life of patients is low, which brings a heavy burden to the family and the country. However, most early gastric cancers can be tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 程昌明
Owner SHANGHAI BIOMEDICAL LAB CO LTD
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