A wavelength dispersion probing system for determining a value of wavelength dispersion and its sign and reducing the trouble and time required for this determination. This wavelength dispersion probing system comprises light sources 10, 12, light attenuators 14, 16, optical multiplexer 18, phase modulator 20, optical amplifiers 22, 26, acoustooptical modulator 24, optical receiver 30. The intensity ratio of two wavelengths is set at 1 to 2 to detect Stokes light or anti-Stokes light included in the return light of an optical fiber 100 by the optical receiver 30, so that wavelength dispersion is probed. The wavelength dispersion is probed by changing the intensity ratio of the two wavelengths to observe the state of the change of the wavelength dispersion, so that the sign of wavelength dispersion is determined by the optical receiver 30.