Methylated biomarkers related to gastric cancer lymph node metastasis detection or combination and application thereof

A technology of biomarkers and lymph node metastasis, which is applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve the problem of limited accuracy of lymph node status assessment in gastric cancer, and achieve accurate clinical diagnosis and guidance The effect of treatment

Active Publication Date: 2022-04-12
ANCHORDX MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Endoscopic ultrasonography, computed tomography (CT), positron emission tomography (PET-CT), etc., have limited accuracy in assessing lymph node status in early gastric cancer

Method used

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  • Methylated biomarkers related to gastric cancer lymph node metastasis detection or combination and application thereof
  • Methylated biomarkers related to gastric cancer lymph node metastasis detection or combination and application thereof
  • Methylated biomarkers related to gastric cancer lymph node metastasis detection or combination and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Biomarker (marker) screening

[0045] 1. Sample source:

[0046] Samples must meet the following requirements: 1) patients with early gastric cancer diagnosed as T1 stage; 2) no distant metastasis or family history; 3) no neoadjuvant therapy; 4) lymphoma, multiple tumors, stump carcinoma and intraepithelial carcinoma Cases with neoplastic changes were excluded; 5) At least three gastrointestinal pathology experts evaluated the tumor stage and lymph node metastasis status. 12 pairs of gastric cancer with lymph node metastasis and 11 pairs of gastric cancer with non-lymph node metastasis, a total of 46 samples, all fresh and frozen tissue samples, the clinical information of the samples is shown in Table 1.1:

[0047]

[0048]

[0049] 2. Experiment, data analysis and biomarker screening:

[0050] The above samples are used to build a library of fresh frozen tissue or FFPE samples. The process and steps are as follows:

[0051] 1. Tissue DNA extraction ...

Embodiment 2

[0127]Using the 12 pairs of gastric cancer lymph node metastasis cancer and adjacent cancer and 11 pairs of gastric cancer non-lymph node metastasis cancer and adjacent cancer described in Example 1, a total of 46 samples, using the 60 biomarkers (markers) screened in Example 1 to establish Divide these 48 samples into 100 times according to the ratio of 7:3, use Random Forest (Random Forest) to model, use Random Forest (Random Forest) to model in the train set in each segmentation, use this model The risk score of each sample is calculated in the test set, and the risk score is compared with the standard diagnosis to obtain the discrimination sensitivity, specificity, AUC, NPV, and PPV of the combination of methylated regions. Specific allocation: Randomly select markers based on 4marker (chr12:42873140, chr8:81789921, chr8:81811441 and chr8:16884489), respectively 4marker (chr12:42873140, chr8:81789921, chr8:81811441, chr8:16884489) 10marker(chr12:42873140、chr8:81789921、chr8...

Embodiment 3

[0130]On the basis of the 60 biomarkers (markers) screened in Example 1, randomly select biomarkers from the 1366 biomarkers (markers) and 1306 biomarkers (markers) except 60 marker and 60 biomarkers (marker) combined into 100 biomarkers (marker) (chr1:2980508, chr1:9054472, chr1:53387866, chr10:15032997, chr10:129535510, chr11:460694, chr12:6729752 、chr12:42873140、chr12:52644274、chr12:107713297、chr13:50707693、chr13:113765062、chr14:48145305、chr15:21968071、chr16:22825741、chr16:31487303、chr16:55358704、chr16:86542314、chr17:6617301、chr19 :4054950、chr19:12876993、chr19:40421554、chr19:46388022、chr19:46388262、chr19:46388316、chr19:46388329、chr19:55593563、chr19:57352008、chr2:99439396、chr2:115420396、chr2:210636540、chr20:43726625 、chr20:54579248、chr20:57429986、chr20:59827184、chr20:59828473、chr20:61472147、chr20:61638536、chr21:22370447、chr4:20254852、chr5:2749179、chr5:140799056、chr5:163754234、chr6:85473377、chr6 :118228600、chr7:330023、chr7:47092638、chr7:56242574、chr7:71801447、chr7:98971536、c...

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Abstract

The invention relates to a methylated biomarker or a combination thereof for detecting gastric cancer lymph node metastasis and non-metastasis and application of the methylated biomarker. The methylated biomarker is selected from at least one of chr12: 42873140, chr8: 81789921, chr8: 81811441 and chr8: 16884489. According to the invention, the appropriate DNA methylation biomarker is screened and used for detecting gastric cancer lymph node metastasis and non-metastasis, and the DNA methylation biomarker can achieve the purpose of accurate prediction. The novel DNA methylation marker developed by the invention can be used for identifying whether the lymph node metastasis of the early gastric cancer occurs or not, can be used for assisting clinical accurate diagnosis and guiding treatment, and also can be used for technical research in laboratories.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a methylation biomarker for detecting gastric cancer lymph node metastasis or its combination and application. Background technique [0002] Gastric cancer (GC) is a malignant tumor and one of the most common malignant tumors in the world. According to the 2020 global cancer statistics data, the incidence and mortality of gastric cancer rank fifth and third respectively, and the incidence rate of men is twice that of women. In China, the incidence of gastric cancer is increasing year by year, and tends to be younger. Its incidence and death rate have jumped to the second and third respectively, ranking first in malignant tumors of the digestive system, and the number of gastric cancers almost accounts for the number of gastric cancers in the world half of. Gastric cancer, as a highly aggressive and heterogeneous malignancy, remains a global health problem. [0003] Cli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12Q1/6869C12N15/11
CPCC12Q1/68C12N15/11C12Q1/6886C12Q1/6869C12Q1/6858
Inventor 王军阮微媚陈志伟
Owner ANCHORDX MEDICAL CO LTD
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