Nano-composite for early diagnosis and treatment of gastric cancer and preparation method of nano-composite

A nanocomposite and early diagnosis technology, which is applied in the fields of polymer materials and medical engineering, can solve problems such as poor targeting, small molecular weight, and fast metabolism, and achieve the goal of controlling drug release, wide application prospects, and improving therapeutic effects Effect

Inactive Publication Date: 2020-05-12
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many research results have shown that the connection of RGD with fluorescent molecules or radionuclides has a certain tumor-targeted imaging function. However, the molecular weight of RGD and fluorescent molecules or radionuclide conjugates is small, the metabolism in the body is fast, and it is easy to be cleared. lead to poor targeting

Method used

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  • Nano-composite for early diagnosis and treatment of gastric cancer and preparation method of nano-composite
  • Nano-composite for early diagnosis and treatment of gastric cancer and preparation method of nano-composite
  • Nano-composite for early diagnosis and treatment of gastric cancer and preparation method of nano-composite

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Comparison scheme
Effect test

Embodiment 1

[0039] An embodiment of the nanocomposite of the present invention, its preparation method comprises the following steps:

[0040] (1) Weigh the indocyanine green of 1 part by weight, add 5 mL of tetrahydrofuran, mix the mixture with distearoylphosphatidylethanolamine-polyethylene glycol 2000-maleimide and weight of 5 parts by weight After mixing 0.5 parts of doxorubicin, sonicate at room temperature for 10 minutes to fully disperse and dissolve distearoylphosphatidylethanolamine-polyethylene glycol 2000-maleimide, indocyanine green and doxorubicin , after mixing, place the round bottom flask on a rotary evaporator, put it in a water bath at 70°C, pump it to a vacuum and then rotate it to evaporate; when the organic solvent evaporates completely, the nanostructure is successfully transferred from the oil phase to the water phase;

[0041] (2) After it is cooled to room temperature, apply an ultrasonic probe for 15 minutes to disperse it; filter through a 220nm filter membrane an...

Embodiment 2

[0046] An embodiment of the nanocomposite of the present invention, its preparation method comprises the following steps:

[0047] (1) Weigh the indocyanine green of 5 parts by weight, add 5mL tetrahydrofuran, mix the mixture with distearoylphosphatidylethanolamine-polyethylene glycol 2000-maleimide and weight of 20 parts by weight After mixing 1 part of doxorubicin, sonicate at room temperature for 10 minutes to fully disperse and dissolve distearoylphosphatidylethanolamine-polyethylene glycol 2000-maleimide, indocyanine green and doxorubicin , after mixing, place the round bottom flask on a rotary evaporator, put it in a water bath at 70°C, pump it to a vacuum and then rotate it to evaporate; when the organic solvent evaporates completely, the nanostructure is successfully transferred from the oil phase to the water phase;

[0048] (2) After it is cooled to room temperature, apply an ultrasonic probe for 15 minutes to disperse it; filter through a 220nm filter membrane and t...

Embodiment 3

[0053] An embodiment of the nanocomposite of the present invention, its preparation method comprises the following steps:

[0054] (1) Weigh the indocyanine green of 2 parts by weight, add 5mL tetrahydrofuran, mix the mixture with the distearoylphosphatidylethanolamine-polyethylene glycol 2000-maleimide and weight of 10 parts by weight After mixing 0.6 parts of doxorubicin in the components, sonicate at room temperature for 10 minutes to fully disperse and dissolve distearoylphosphatidylethanolamine-polyethylene glycol 2000-maleimide, indocyanine green and doxorubicin , after mixing, place the round bottom flask on a rotary evaporator, put it in a water bath at 70°C, pump it to a vacuum and then rotate it to evaporate; when the organic solvent evaporates completely, the nanostructure is successfully transferred from the oil phase to the water phase;

[0055] (2) After it is cooled to room temperature, apply an ultrasonic probe for 15 minutes to disperse it; filter through a 22...

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Abstract

The invention discloses a nano-composite for early diagnosis and treatment of gastric cancer and a preparation method of the nano-composite. The nano-composite has a core-shell structure formed by anamphiphilic copolymer, wherein an inner core layer is formed by a distearoyl phosphatidyl ethanolamine fragment, an outer shell layer is formed by a polyethylene glycol fragment, the inner core layerwraps indocyanine green and a hydrophobic gastric cancer chemotherapeutic drug, and the surface of the outer shell layer is connected with RGD short peptides. The nano-composite preparation integratestargeted gastric cancer treatment and fluorescence imaging tracing, targeted aggregation imaging on gastric cancer focus can be implemented, drug release is controlled, the drug concentration of a targeted site is increased, the drug action time is prolonged, and the nano-composite has a wide application prospect in targeted diagnosis and treatment and drug delivery of early gastric cancer.

Description

technical field [0001] The invention relates to the technical fields of polymer materials and medical engineering, in particular to a nanocomposite used for early diagnosis and treatment of gastric cancer and a preparation method thereof. Background technique [0002] Gastric cancer is a common malignant tumor in my country, with more than 1 million new cases each year, and more than 80% of the cases are diagnosed in the middle and advanced stages, and the annual death rate is as high as 700,000. Therefore, the diagnosis and treatment of gastric cancer and colorectal cancer have become an urgent need. solved problem. At present, endoscopic diagnosis of some early lesions is difficult, and endoscopic resection is also difficult to grasp the margins. The treatment of gastric cancer is still generally treated with oral or injected anticancer chemotherapy drugs (chemotherapy). However, the specific aggregation of drugs at the tumor site is not effective, and there is a lack of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K31/704A61K31/513A61K31/337A61K33/243A61K9/107A61K47/10A61K47/18A61K47/42A61P35/00
CPCA61K49/0034A61K49/0054A61K49/0052A61K49/0082A61K49/0093A61K31/704A61K31/513A61K31/337A61K33/243A61K9/107A61K47/10A61K47/18A61K47/42A61P35/00
Inventor 魏波郑晓明邵军梁荣朴蔡梓凯
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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