Major QTL site for flowering time-related traits of brassica napus, SNP molecular markers and application

A Brassica napus, molecular marker technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc. The effect of large-scale promotion of application and accelerated process

Active Publication Date: 2019-12-20
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0004] In the prior art, Zu Feng et al. (Journal of Southern Agricultural Sciences, 2019) pointed out that the flowering period of rapeseed is highly positively correlated with the growth period, which is an important indicator for the selection of early maturity traits of rapeseed. However, as quantitative traits, the two are controlled by multiple genes and interact with the environment. Significant effect, it is difficult to achieve direct selection without the assistance of molecular markers

Method used

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  • Major QTL site for flowering time-related traits of brassica napus, SNP molecular markers and application
  • Major QTL site for flowering time-related traits of brassica napus, SNP molecular markers and application
  • Major QTL site for flowering time-related traits of brassica napus, SNP molecular markers and application

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Embodiment 1

[0030] The mensuration of the phenotype of embodiment 1 Brassica napus anthesis trait

[0031] 1. Determination of Anthesis Phenotypes in Associated Populations

[0032] (1) A natural population of 324 high-generation lines of Brassica napus from all over the world was formed, and a 3-year and 2-point investigation of flowering period traits was completed at the Yangluo Experimental Base of Wuhan City and the Experimental Base of Yangzhou Academy of Agricultural Sciences.

[0033] (2) The seedlings were fixed by direct seeding, the row spacing was 33 cm, the plant spacing was 15 cm, 4 rows in each plot, and three replicates were randomly designed. Protective rows were planted around the test material fields.

[0034] (3) Flowering period: the number of days obtained by subtracting the planting date from the date when 25% of the plants in the whole area begin to flower.

[0035] (4) Use the BLUP method (http: / / www.extension.org / pages / 61006) to integrate multi-year multi-point...

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Abstract

The invention provides a major QTL site for flowering time-related traits of brassica napus. The major QTL site is located between the 6143445th base and the 7287910th base of an A02 chromosome of thebrassica napus, and preferably has a contribution rate of 15.43 percent for the flowering time-related traits of the brassica napus. The major QTL site is closely linked with a first SNP molecular marker which is located on the 6143445th base and is A or C, and the mutation causes polymorphism; the major QTL site is closely linked with a second SNP molecular marker which is located on the 7287910th base and is A or G, and the mutation causes polymorphism; and the major QTL site is closely linked with a peak SNP molecular marker which is located on the 6330026th base and is C or G, and the mutation causes polymorphism. The invention further provides related SNP molecular markers and application. The major QTL site for the flowering time-related traits of brassica napus has a high contribution rate for the flowering time-related traits of the brassica napus, has a key effect on regulation of the flowering time-related traits of brassica napus, can be used as map-based cloning and molecular marker assisted selection and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of molecular biology and rapeseed breeding, in particular to the technical field of flowering stage traits of Brassica napus, in particular to SNP molecular markers closely linked to major QTL loci of flowering stage traits of Brassica napus and its application. Background technique [0002] Rapeseed (Brassica napus) is an important oil crop in the Brassicaceae family. napus is the main oil crop widely planted in the world, and it is also the only winter oil crop in my country. Therefore, the country regards the development of rapeseed production as the focus of ensuring the safe supply of edible oil, which has important practical significance. Brassica napus in my country has become the main cultivated variety due to its characteristics of disease resistance, high yield, wide adaptability, and strong retrograde tolerance. Rapeseed production can be increased mainly through three ways: high rapeseed yield p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 刘胜毅张园园唐敏强刘越英程晓辉黄军艳童超波董彩华
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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