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Soybean GmFTL3 protein and soybean GmFTL5 protein as well as applications thereof

A soybean and protein technology, applied in the field of genetic engineering, can solve the problems of changing plant sensitivity and flowering time, and achieve the effect of strong effect and promoting plant flowering

Active Publication Date: 2011-08-10
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Changing plant sensitivity to photoperiod and flowering time by modulating soybean flowering gene expression has also not been reported

Method used

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  • Soybean GmFTL3 protein and soybean GmFTL5 protein as well as applications thereof
  • Soybean GmFTL3 protein and soybean GmFTL5 protein as well as applications thereof
  • Soybean GmFTL3 protein and soybean GmFTL5 protein as well as applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Cloning of soybean flowering genes GmFTL3 and GmFTL5

[0025] Using the forward primer 5'-ATGCCTAGTGGAAGTAGGGAT-3' and the reverse primer 5'-TTAGTATAACCTCCCTTCCACC-3' and the forward primer 5'-AACCAATTGTAAAGTAGTTTCCA-3' and the reverse primer 5'-GTCCCTTATTTTATAAAGATCAAAA-3' from soybean Kennong 18 (Glycine max L.Kennong 18) were cloned and sequenced to obtain GmFTL3 and GmFTL5 genes, the gene sequences of which are shown in SEQ ID NO.3 and SEQ ID NO.4; the amino acid sequences of proteins encoded by them are shown in SEQ ID NO.1 and Shown in SEQ ID NO.2.

[0026] The PCR reaction program was: 95°C 5min pre-denaturation, 95°C 30S, 55°C 35S, 72°C 1min, 25 cycles, 72°C 10min extension.

Embodiment 2

[0027] Example 2 Amino Acid Sequence Analysis of Proteins Encoded by Soybean Flowering Genes GmFTL3 and GmFTL5

[0028] The homology between the GmFTL3 and GmFTL5 protein sequences of the soybean flowering gene is greater than 90.9%, and the C-terminal functional domain is highly homologous to Arabidopsis, but there are important differences, such as figure 1 shown. The 131st amino acid residue of soybean GmFTL3 and GmFTL5 protein is glutamic acid (E), the 150th amino acid residue is leucine (L), and the corresponding parts of Arabidopsis are glutamine (Q) and isoleucine amino acid (I). These differences lead to higher activity of GmFTL3 and GmFTL5 than Arabidopsis FT.

Embodiment 3

[0029] Example 3 Cloning vectors of soybean flowering genes GmFTL3 and GmFTL5

[0030] The PCR product that amplifies and obtains from embodiment 1 is cloned directly according to the TA cloning method image 3on the indicated pGWCm vector. Firstly, the pGWCm vector was hydrolyzed with Ahd I endonuclease, and then the digested product was recovered with a gel recovery kit to obtain the T vector. Then the PCR product and the T vector were ligated at 16° C., the ligated product was transformed into Escherichia coli DH5α, amplified therein, positive clones were screened and sequenced.

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PUM

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Abstract

The invention discloses a soybean GmFTL3 protein and a soybean GmFTL5 protein which have the amino acid sequence shown in SEQ ID No.1 and the amino acid sequence SEQ ID No.2 respectively or the sequences which are separately obtained through replacing, deleting or adding one or more of amino acid residues and have the same functions. The GmFTL3 and GmFTL5 which are capable of overexpressing soybean flowering genes are used for obviously promoting the flowering of plant (Arabidopsis thaliana), thus the flowering time can be shortened and the growth period can be shortened. The proteins can be used for solving the problem of flowering asynchronism in the hybridization breeding process, the growth period control problems of various crops, vegetable, fruits and flowers, the problem of photoperiod sensitivity and the introduction problem.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to two soybean flowering genes, their encoded proteins and their application in the regulation of plant photoperiod and flowering time. Background technique [0002] Soybean is one of the important crops, and it is an important source of plant protein, edible oil, biodiesel, and secondary metabolites such as isoflavones and lecithin. Because soybean is a short-day plant, flowering is strictly controlled by photoperiod, so excellent varieties in different regions cannot be introduced to each other, and the growth period is also restricted by environmental photoperiod (Zhang et al., 2008). If the sensitivity of soybean to photoperiod can be reduced, and the limitation of soybean flowering to photoperiod can be broken, the problem of soybean introduction can be solved, so as to realize the mutual exchange of high-quality varieties in various regions, enrich excellent germplasm resour...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00A01H5/02
Inventor 傅永福张晓玫
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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