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Precursor gene of hickory micro159a and application thereof to advancing of plant flowering

A technology of hickory and its precursor, applied in the field of genetic engineering, can solve the problems such as the unknown function of hickory, and achieve the effect of early flowering and fruiting, and shortened childhood

Inactive Publication Date: 2018-11-23
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functional role of hickory miR159a is unknown

Method used

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  • Precursor gene of hickory micro159a and application thereof to advancing of plant flowering
  • Precursor gene of hickory micro159a and application thereof to advancing of plant flowering
  • Precursor gene of hickory micro159a and application thereof to advancing of plant flowering

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Embodiment Construction

[0021] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0022] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] 1. Materials

[0025] 1.1 Experimental materials

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Abstract

The invention discloses a precursor gene of hickory micro159a. The sequence of the precursor gene is: (a) a nucleotide sequence shown as SEQ ID NO:1, or (b) a nucleotide sequence which has at least 70percent, at least 75 percent, at least 80 percent, at least 85 percent, at least 90 percent, at least 95 percent, at least 96 percent, at least 97 percent, at least 98 percent or at least 99 percentof homology with the nucleotide sequence shown as SEQ ID NO:1 and can be used for generating the hickory micro159a after cutting. The application of the precursor sequence of the hickory micro159a isexpected to advance the plant flowering artificially. Specifically, the application of the hickory micro159a is expected to regulate the flowering period of hickory, shorten the childhood of hickory and advance flowering and fruiting.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a precursor gene of pecan miR159a and its application in early flowering of plants. Background technique [0002] miRNA is an endogenous, non-coding RNA with a length of about 20-24nt. In recent years, it has been found that the regulatory mechanism composed of miRNA and its target genes widely exists in the regulatory network of plant development and stress response. In this process, miRNA, as a key factor, degrades target genes or inhibits target gene expression, and participates in plant growth and development. Genome duplication events generate duplicate miRNA clusters, and miRNA precursors in flowering plants have the potential to encode multiple mature miRNAs, whether identical or different. [0003] The miR159 family mainly plays an important role in the GA signaling pathway, and miR159 is one of the eight highly conserved miRNAs that have been co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82C12N5/10A01H5/00A01H6/20A01H6/82
CPCC12N15/113C12N15/827C12N2310/141
Inventor 王正加舒李露孙志超
Owner ZHEJIANG FORESTRY UNIVERSITY
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