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A new grape variety and rapid technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of large manpower, material and financial resources, high degree of heterozygosity, and low frequency of appearance of desired traits, and achieve The effect of stable traits and shortened childhood
Pending Publication Date: 2019-11-05
HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI
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Moreover, grapes, like other asexually propagated woody fruit trees, have a high degree of heterozygosity in genetics, a large segregation range of hybrid offspring, and a low frequency of required traits, which brings great difficulties to breeding work and takes a long time and effort. Greater manpower, material and financial resources
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specific Embodiment approach 1
[0055] Embodiment 1: A method for rapidly cultivating a new grape variety of the present embodiment is carried out according to the following steps:
[0056] 1. Hybridization:
[0057] 1.1. Collection of male parent pollen
[0058] Collect pollen from the rose-scented grape variety Morissa;
[0059] 1.2, emasculation of the female parent
[0060] 50 spikes of Shirinag grapes were detasselled and bagged;
[0061] 1.3. Hybrid pollination
[0062] Crossing Baimunag and Morisa, using the pollen of the male parent grapes to pollinate the detasselled female parent grapes, and repeat pollination once every other day;
[0063] 2. Hybrid ovule and seedling cultivation
[0064] 2.1. Embryo extraction
[0065] At the beginning of July in the first year, the fruit is sterilized, and the fruit stalk is cut off from the ear of the fruit, and marked separately. The fruit is washed twice with tap water and sterile water, dried for 10 minutes, and the fruit is soaked in 75% alcohol and sti...
specific Embodiment approach 2
[0104] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 2.4, the endosperm is placed in a light incubator for in vitro culture for 10 to 20 days. Others are the same as the first embodiment.
[0105] The general incubation time is 15 days.
specific Embodiment approach 3
[0106] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the culture time in the tissue culture room in step 3.1 is 20 days. Others are the same as those in Embodiment 1 or 2.
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Abstract
The invention relates to a method for rapidly cultivating a new grape variety. The method for cultivating the new grape variety with excellent traits aims at shortening the crossbreeding time. According to the method, by combining the characteristics of high-quality fruits of a variety with Xinjiang native characteristics, namely white Munake grapes, and integrating the special rose fragrance of aMelissa grape variety and a flame seedless grape variety, the technical problems of long periodicity and low occurrence frequency of required traits by means of grape crossbreeding methods are solved, and the new grape dominant variety is invented. The method adopts a general crossing technology and comprises the technical steps of crossing, wherein a, pollen of male parents is collected, b, emasculation of female parents is conducted, and c, crossing pollination is conducted; cross ovule and seedling culture, wherein d, ovules are taken out, e, the ovules are cultured, f, the ovules are cut,g, the ovules are subjected to germination culture, and h, seedlings are cultured; propagation expanding and transplanting, wherein i, propagation expanding is conducted, j, seedling hardening is conducted, k, transplanting is conducted, and l, top grafting is conducted; fruit quality identification. The method uniquely adopts a high-nutrition seedling stage management mode, integrates a breedingmeans combining ovule rescue and top grafting and shortens the cycle of traditional grape cross breeding, and the cultivated new grape variety has target traits.
Description
technical field [0001] The present invention relates to a method for cultivating new grape varieties. Background technique [0002] Grape has always been one of the most important fruits in the world, and its output and area are in the forefront of the world's fruits. According to statistics from the Ministry of Agriculture, by the end of 2016, the total area of grape cultivation in my country was 809,600 hectares, ranking second in the world; the output reached 13.745 million tons, ranking first in the world. Xinjiang is the largest grape-producing area in the country. In 2017, the grape cultivation area reached 143,000 hectares and the output was more than 2.6 million tons. The cultivation area and output accounted for 18.79% and 19.47% of the country's respectively. It is one of the most advantageous fruit trees in Xinjiang. Breeding high-quality early-maturing, storable, and fragrant varieties suitable for open field viticulture is always the direction of grape breedi...
Claims
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