Method for rapidly cultivating new grape variety
A new grape variety and rapid technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of large manpower, material and financial resources, high degree of heterozygosity, and low frequency of appearance of desired traits, and achieve The effect of stable traits and shortened childhood
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specific Embodiment approach 1
[0055] Embodiment 1: A method for rapidly cultivating a new grape variety of the present embodiment is carried out according to the following steps:
[0056] 1. Hybridization:
[0057] 1.1. Collection of male parent pollen
[0058] Collect pollen from the rose-scented grape variety Morissa;
[0059] 1.2, emasculation of the female parent
[0060] 50 spikes of Shirinag grapes were detasselled and bagged;
[0061] 1.3. Hybrid pollination
[0062] Crossing Baimunag and Morisa, using the pollen of the male parent grapes to pollinate the detasselled female parent grapes, and repeat pollination once every other day;
[0063] 2. Hybrid ovule and seedling cultivation
[0064] 2.1. Embryo extraction
[0065] At the beginning of July in the first year, the fruit is sterilized, and the fruit stalk is cut off from the ear of the fruit, and marked separately. The fruit is washed twice with tap water and sterile water, dried for 10 minutes, and the fruit is soaked in 75% alcohol and sti...
specific Embodiment approach 2
[0104] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 2.4, the endosperm is placed in a light incubator for in vitro culture for 10 to 20 days. Others are the same as the first embodiment.
[0105] The general incubation time is 15 days.
specific Embodiment approach 3
[0106] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the culture time in the tissue culture room in step 3.1 is 20 days. Others are the same as those in Embodiment 1 or 2.
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