The invention discloses a method for extracting and separating tannins components of immature fruits of
medicine terminalia. The method includes the steps of firstly, taking medicinal materials of the immature fruits of the
medicine terminalia, crushing the medicinal materials, using an
acetone solution as an extracting agent, adding the
acetone solution with the volume 2-6 times of the volume of the medicinal materials, performing extraction for 2-4 times by using a
room temperature immersion method, combining extract liquids and concentrating the extract liquids into extractum; secondly, extracting the extractum by using a small amount of
ethyl acetate for a plurality of times, combining
ethyl acetate extract liquids, and concentrating the
ethyl acetate extract liquids until the extract liquids are free of ethyl acetate odors; and thirdly, using methyl
alcohol to redissolve concentrated substances, adding a macroreticular resin column, using a methyl
alcohol aqueous solution for eluting the substances and concentrating and
drying eluent to obtain tannins extractives of the immature fruits of the
medicine terminalia. The tannins component content of the extractives is larger than 80%, a method for determining
tannin contents refers to a phosphomolybdium
tungstic acid-
casein method for determining the
tannin contents, and the phosphomolybdium
tungstic acid-
casein method is recorded in the first part addendum XB of
Chinese pharmacopoeia 2010 edition. The method for extracting and separating the tannins components of the immature fruits of medicine terminalia is small in
solvent consumption, high in efficiency, high in content and applicable to industrial
mass production.