Method for extracting and separating tannins components of immature fruits of medicine terminalia

A separation method and technology of tannins are applied in the directions of plant/algae/fungus/moss components, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems of time-consuming, large consumption of solvents, troublesome operation, etc. Achieve high efficiency, high tannin content and low consumption

Inactive Publication Date: 2012-11-07
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] CN200510042885.2 discloses the preparation method and application of effective parts extracted from green fruit for bacterial and viral infectious diseases. Three preparation methods are mainly used to extract the phenolic compounds in green fruit for bacterial and viral infections, but Among them, the decoction method and the reflux method both use heating, which is easy to heat and decompose the tannin components in the green fruit; while the other percolation method consumes a large amount of solvent, takes a long time, and is troublesome to operate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Get 450g of green fruit medicinal material, pulverize, add acetone aqueous solution, soak and extract continuously three times at room temperature, add about 1.25L of acetone solution of 5 times of volume (v:v) for the first time and extract for 48 hours; Add 1 L of 80% acetone solution to extract each time for 24 hours, combine the extraction liquids, filter, and concentrate the filtrate under reduced pressure at 45°C to obtain an extract. Then extract with ethyl acetate, add 300ml of ethyl acetate each time, extract 5 times, combine the ethyl acetate extracts, concentrate under reduced pressure at 40°C, and concentrate until there is no ethyl acetate odor. Completely redissolve, apply Diaion HP-20 macroporous adsorption resin (the resin is first activated), and use 20%, 40% and 60% (v:v) methanol aqueous solution to elute successively, collect 60% methanol aqueous solution eluate, The obtained purified liquid was concentrated under reduced pressure at 40° C., then pla...

Embodiment 2

[0018] Take 500g of the green fruit medicinal material, grind it into fine powder, add 1L of 85% acetone aqueous solution three times in a row, extract for 24 hours each time, combine the extracts, filter, and concentrate the filtrate at 40°C under reduced pressure to obtain an extract. Then extract with ethyl acetate, 250ml each time, extract 5 times, combine the ethyl acetate extracts, concentrate under reduced pressure at 40°C until there is no ethyl acetate odor, redissolve the concentrate in 100ml of methanol, and adsorb on SEPABEDS-SP825 macropores Resin (resin activated first), eluted with 30%, 50%, and 70% (v; v) methanol aqueous solution in turn, collected 50% methanol aqueous solution eluate to obtain a purified solution, concentrated under reduced pressure at 40 ° C, and then placed In a constant temperature and reduced pressure drying oven, dry under reduced pressure to obtain 200 g of the tannin extract of the green fruit with a tannin content of 89.6%.

Embodiment 3

[0019] Embodiment three: prepare the capsule of green fruit tannin by technical method known in the art

[0020] Get 100g of the tannin extract of the green fruit of Example 1, use starch and dextrin as fillers, granulate with 3% starch slurry as a binding agent, add an appropriate amount of magnesium stearate, fill capsules, and each capsule contains Green fruit tannin 100mg, ready to pack.

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PUM

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Abstract

The invention discloses a method for extracting and separating tannins components of immature fruits of medicine terminalia. The method includes the steps of firstly, taking medicinal materials of the immature fruits of the medicine terminalia, crushing the medicinal materials, using an acetone solution as an extracting agent, adding the acetone solution with the volume 2-6 times of the volume of the medicinal materials, performing extraction for 2-4 times by using a room temperature immersion method, combining extract liquids and concentrating the extract liquids into extractum; secondly, extracting the extractum by using a small amount of ethyl acetate for a plurality of times, combining ethyl acetate extract liquids, and concentrating the ethyl acetate extract liquids until the extract liquids are free of ethyl acetate odors; and thirdly, using methyl alcohol to redissolve concentrated substances, adding a macroreticular resin column, using a methyl alcohol aqueous solution for eluting the substances and concentrating and drying eluent to obtain tannins extractives of the immature fruits of the medicine terminalia. The tannins component content of the extractives is larger than 80%, a method for determining tannin contents refers to a phosphomolybdium tungstic acid-casein method for determining the tannin contents, and the phosphomolybdium tungstic acid-casein method is recorded in the first part addendum XB of Chinese pharmacopoeia 2010 edition. The method for extracting and separating the tannins components of the immature fruits of medicine terminalia is small in solvent consumption, high in efficiency, high in content and applicable to industrial mass production.

Description

technical field [0001] The invention relates to a method for extracting and purifying components of traditional Chinese medicines, in particular to extracting and purifying tannin components from Xiqingguo (also known as Tibetan Qingguo and Tibetan Qingguo). Background technique [0002] The Chinese Pharmacopoeia records that the medicinal material Xiqingguo comes from the dried young fruit of Terminalia chebula Retz. Its nature is bitter, sour, astringent, flat, and returns to the lung and large intestine meridians. It has the effects of clearing away heat, promoting body fluid and detoxification, and it is mainly used clinically for the treatment of pneumonia, laryngitis, tonsillitis, bacillary dysentery and acute enteritis. Its main chemical components are tannin phenolic acids, triterpenoids and volatile oils, among which the content of tannin components is closely related to its pharmacological activity and is one of the important effective components. Therefore, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P11/00A61P11/04A61P31/04A61P1/12A61P1/00A61K125/00
Inventor 刘松青刘芳刘耀王玲艳
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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