A kind of ultrasonic-assisted bio-enzymatic method for extracting Acacia chinense extract
A bio-enzymatic, ultrasonic-assisted technology, applied in chemical instruments and methods, natural resin technology, natural resin refining, etc., to achieve the effects of high tannin content, promoting cell wall breakage, and increasing solubility
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Embodiment 1
[0027] A method for ultrasonically assisted bio-enzymatic extraction of acacia extract, comprising the steps of:
[0028] (1) the Acacia acacia bark is cleaned with water, dried, and pulverized to 100 mesh to obtain Acacia acacia bark powder (the tannin content is 32.7%);
[0029] (2) Take 100kg of Acacia blackwood bark powder and put it into ultrasonic countercurrent extraction equipment, then add water 10 times the weight of the bark powder, adjust the pH to 5.5 with citric acid, and then add cellulose with a mass ratio of 3:2:1 Enzyme, pectinase and xylanase, its addition is 1.2% of the mass of Acacia blackwood bark powder, ultrasonic countercurrent extraction is 1.5h at a temperature of 60°C, the temperature is raised to 70°C, ultrasonic countercurrent extraction is 1.0h, and then Raise the temperature to 85°C, perform ultrasonic countercurrent extraction for 1.0 h, and filter to obtain an extract containing tannin extract;
[0030] (3) Add 8g of EDTA, 45g of attapulgite ...
Embodiment 2
[0034] A method for ultrasonically assisted bio-enzymatic extraction of acacia extract, comprising the steps of:
[0035] (1) the Acacia acacia bark is cleaned with water, dried, and pulverized to 100 mesh to obtain Acacia acacia bark powder (the tannin content is 32.7%);
[0036] (2) Take 100kg of Acacia blackwood bark powder and put it into ultrasonic countercurrent extraction equipment, then add water 12 times the weight of the bark powder, adjust the pH to 5.0 with acetic acid, and then add cellulase with a mass ratio of 2:1:1 , pectinase and xylanase, the addition of which is 1.0% of the mass of Acacia blackwood bark powder, ultrasonic countercurrent extraction at a temperature of 60°C for 2h, warming up to 75°C, ultrasonic countercurrent extraction for 1.5h, and then heating up to 80°C, ultrasonic countercurrent extraction for 0.5h, filtered to obtain the extract;
[0037] (3) Add 10g of EDTA, 40g of attapulgite and 4g of oxalic acid to each liter of the extract, let it...
Embodiment 3
[0041] A method for extracting acacia extract from black wood with ultrasonic-assisted biological enzyme method, comprising the steps of:
[0042](1) the Acacia acacia bark is cleaned with water, dried, and pulverized to 100 mesh to obtain Acacia acacia bark powder (the tannin content is 35.2%);
[0043] (2) Take 100kg of Acacia blackwood bark powder and put it into ultrasonic countercurrent extraction equipment, then add water 9 times the weight of the bark powder, adjust the pH to 4.5 with phosphoric acid, and then add cellulase with a mass ratio of 3:1:1 , pectinase and xylanase, the addition of which is 0.8% of the mass of Acacia blackwood bark powder, ultrasonic countercurrent extraction at a temperature of 50°C for 2h, warming up to 70°C, ultrasonic countercurrent extraction for 1.5h, and then heating to 85°C, ultrasonic countercurrent extraction for 1 hour, and filter to obtain the extract;
[0044] (3) Add 8g of EDTA, 50g of attapulgite and 3g of oxalic acid to each l...
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