Lotus corniculatus LcMYB5 gene and application thereof

Abstract

The invention provides a lotus corniculatus LcMYB5 gene. A nucleotide sequence of the lotus corniculatus LcMYB5 gene is selected from at least one of sequences as shown in SEQ ID No: 3-8. The invention further provides application of the lotus corniculatus LcMYB5 gene in improvement on the tannin content of plant leaves. According to the invention, the new key regulatory gene LcMYB5 for regulating tannin biosynthesis in lotus corniculatus is identified for the first time. The lotus corniculatus LcMYB5 gene provides a new candidate gene for cultivating forage grass varieties with high-content tannin in leaves, and provides a new way for cultivating new forage grass varieties with anti-tympanites quality; the gene can be used for further genetic improvement to increase the leaf tannin content in lotus corniculatus; the gene can also be used for carrying out genetic improvement on forage grass without accumulation of tannin on leaves or with low tannin content to increase the tannin content so as to prevent or slow down the tympanites, thereby reducing the economic loss.

Description

technical field [0001] The invention relates to LcMYB5 gene of japonicus japonicus and application thereof, in particular to application of LcMYB5 gene of japonicus japonicus in regulating tannin content, and belongs to the technical field of genetic engineering application. Background technique [0002] Tannins (Proanthocyanidins) are flavonoid secondary metabolites with important activities in plants. It not only plays an important role in the regulation of seed dormancy, lifespan and germination, but also participates in the regulation of biotic and abiotic stresses in plants. In animal husbandry, tannins in pasture can also inhibit the growth of rumen methanogens in ruminants, reduce the release of methane, inhibit the growth of gastrointestinal parasites, improve animal fertility, and promote fetal development, wool production and lactation. Appropriate amount Tannins are also effective in preventing the often fatal bloat disease in ruminants such as cattle and sheep. ...

AI Technical Summary

Problems solved by technology

However, there are few studies on the synthesis of leaf tannins in japonicus japonicus. Only in 1983, Ross et al. conducted a population analysis on the leaf tannins of nearly 200 different japonicus japonicus varieties, and found that the content of tannins in the leaves was polymorphic. And it is controlled by single-gene dominant inheritance, but the gene has not been studied in depth

Therefore, the molecular mechanism of leaf tannin biosynthesis in J. japonicus remains unclear, hampering further breeding efforts to increase leaf tannin content through genetic improvement.

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