Method utilizing liquid phase capture to carry out gene typing of large yellow croaker genome
A technology of genotyping and large yellow croaker, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of high cost, difficulty in application, and complicated gene chip design, so as to improve efficiency, specificity, Effects of High Efficiency Genome Typing Technology
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] In the embodiment of the present invention, a liquid-phase capture method for whole-genome genotyping of large yellow croaker:
[0024] 1. Resequencing data using large yellow croaker
[0025] In view of the fact that the above-mentioned individuals used for genome sequencing are highly homozygous individuals with few heterozygous sites / SNP sites, 100 large yellow croakers (50 male and 50 male) were selected from different breeding groups in September 2012 , Genome resequencing was carried out by constructing a 300bp small fragment library. The two groups of resequencing obtained a total of 20Gb of raw data, and the average sequencing depth reached 26.8.
[0026] 2. Determine the capture site, probe design
[0027] The sequencing results were compared to the above draft genome for SNP marker mining. A total of 12,161,573 SNP sites with a sequencing depth greater than 5X, 505,286 on exons, 4,949,058 on introns, and 5,135,488 between genes were obtained. According to ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com