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Molecular marks closely linked to glomerella leaf spot resistant genetic loci of apple and application

A gene locus and molecular marker technology, applied in the field of molecular markers and applications, can solve problems such as long breeding cycle

Active Publication Date: 2017-03-22
QINGDAO AGRI UNIV
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Problems solved by technology

Traditional breeding cycles are long due to the long childhood of apple hybrid trees

Method used

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  • Molecular marks closely linked to glomerella leaf spot resistant genetic loci of apple and application
  • Molecular marks closely linked to glomerella leaf spot resistant genetic loci of apple and application
  • Molecular marks closely linked to glomerella leaf spot resistant genetic loci of apple and application

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Experimental program
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Effect test

Embodiment Construction

[0044] 1 Materials and methods

[0045] 1.1 Extraction of genomic DNA and construction of anti-sensitivity DNA pool

[0046] 207 'Golden Delicious'×'Fuji' F. 1 The hybrid population and 50 field cultivars and strains (the 50 field cultivars and strains are listed in Table 2) were used as test materials. After artificial in vitro inoculation identification, there were 93 disease-resistant plants and 114 susceptible plants in the hybrid population. Among the varieties (lines), there were 33 disease-resistant plants and 17 susceptible plants.

[0047] Take 0.2 g of young leaves from each plant, freeze them quickly in liquid nitrogen, and store them at -70°C. Refer to the CTAB method of Doyle and Doyle (1987) and Cullings (1992) to extract genomic DNA, and make improvements. Use a NanoDrop 2000 spectrophotometer to measure the purity and concentration of DNA, and dilute the concentration to 10ng·μl -1 .

[0048] According to the requirements of the BSA analysis method, the D...

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Abstract

The invention provides molecular marks closely linked to glomerella leaf spot resistant genetic loci of an apple and an application of the molecular marks. The molecular marks include 6 SNP marks and 5 InDel marks, which are SNP3955, SNP4236, SNP4257, SNP4299, SNP4336, SNP4432, InDel4199, InDel4227, InDel4254, InDel4305 and InDel4334 respectively. In addition, the invention provides a mark screening method and the application of the marks. SNP and InDel marks on two sides of an Rgls gene are selected out in combination with a preliminary locating result of the Rgls gene (located between SSR marks S0304673 and S0405127) by utilizing SNP and InDel marks developed through a whole genome re-sequencing technology; 18 SNP loci and 30 InDel loci are analyzed through an HRM curve analysis technology; and 6 SNP marks and 5 InDel marks are screened out to be closely linked to the loci of the Rgls gene.

Description

technical field [0001] The invention belongs to the technical field of apple breeding, and specifically relates to a molecular marker closely linked to an apple anthracnose-resistant leaf blight gene locus and its application. Background technique [0002] Apple anthracnose leaf blight (Glomerella Leaf Spot, GLS) is a highly prevalent fungal disease, which can cause a large number of leaves to dry and fall off in a short period of time, causing secondary flowering in severe cases, and also infecting fruits and causing necrotic spots (Leite et al. 1988; González & Sutton, 1999; González, 2003; Wang et al. 2012; Song Qing et al. 2012). It is difficult to effectively control the disease with drugs. Breeding disease-resistant varieties is an effective way to solve this problem. Due to the long childhood of apple hybrid trees, the traditional breeding cycle is very long. Therefore, the use of DNA molecular markers for early selection is of great significance to improve the eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘源霞兰进好戴洪义张玉刚祝军
Owner QINGDAO AGRI UNIV
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