Method for identifying quantitative trait loci (QTL) and genes related to tender skin colors of cucumbers

Abstract

The invention discloses a method for identifying quantitative trait loci (QTL) and genes related to the tender skin colors of cucumbers. The method comprises the steps of (1) carrying out hybridization on a female parent and a male parent to obtain F1 for constructing recombinant inbred lines (RILs); (2) identifying the tender skin colors of all plants and carrying out statistical analysis of data; (3) extracting genome DNAs of all the RIL plants and the parents thereof; (4) carrying out genome-wide resequencing on the genome DNAs of the parents and the RIL plants, and carrying out single nucleotide polymorphism (SNP) mutation detection and genotyping analysis; (5) determining the LOD threshold of all phenotypes and QTL segments corresponding to same; (6) comparing the SNP detection results in the QTL segments with a cucumber reference genome, searching for related genes, and performing gene function annotation. The method provided by the invention has the advantages that the method adopts a high-throughput sequencing method to perform genotype identification on self-created high generation RILs to obtain a molecular marker number and a high-density linkage map which can notcannotbe reached by the traditional method, so that a support is provided for efficient and accurate identification of the genes related to the tender skin colors of the cucumbers.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a method for identifying QTL and genes related to cucumber tender peel color. Background technique [0002] Cucumber is one of the main vegetables cultivated in open fields and facilities in my country. Because of its delicate flavor, crisp taste and rich nutrition, it is deeply loved by consumers. Cucumber uses the tender fruit as an edible organ, and it is suitable for fried food or raw food in cold salad. Therefore, the color of its commercial fruit is not only the main factor to attract consumers, but also the main target traits that breeders focus on researching and improving. [0003] Many previous studies on fruit color of horticultural crops have shown that peel color is a quantitative trait, and the inheritance of fruit color will show different dominance and recessiveness with different crops and their hybrid combinations. Due to the different con...

AI Technical Summary

Problems solved by technology

The above-mentioned predecessors used AFLP and SSR markers to genetically analyze cucumber tender peel color from the perspective of quality traits. Although a single marker that is more closely linked was obtained, most of the markers were far away and did not predict related genes.

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