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A method for identifying QTLs and genes associated with cucumber tender peel color

A cucumber and peel technology, applied in the field of molecular biology, can solve the problems of long marker distance, unpredicted genes, etc.

Active Publication Date: 2022-05-20
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The above-mentioned predecessors used AFLP and SSR markers to genetically analyze cucumber tender peel color from the perspective of quality traits. Although a single marker that is more closely linked was obtained, most of the markers were far away and did not predict related genes.

Method used

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  • A method for identifying QTLs and genes associated with cucumber tender peel color
  • A method for identifying QTLs and genes associated with cucumber tender peel color
  • A method for identifying QTLs and genes associated with cucumber tender peel color

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Embodiment 1

[0023] Embodiment 1: A kind of identification and the method for QTL and gene relevant with cucumber tender peel color provided by the present invention comprise the following steps, (1) select female parent and male parent, female parent and male parent are crossed to obtain F1, through single grain The recombinant inbred line RIL was constructed by the traditional method, and the female parent, male parent, and RIL strain were planted in the greenhouse until tender fruit grew; for example, the advanced inbred line 1613 was used as the female parent, which is a strong female with green peel and The thorns are dense, the thorns are white, the melon is stick-shaped, and long; the male parent of the advanced inbred line JD7 is a strong male, the peel is white, the thorns are sparse, and the melon is cylindrical and short. The two parents were crossed to obtain F1, and the recombinant inbred line (RIL, F13) was constructed by the method of single seed transmission. The test popula...

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Abstract

A method for identifying QTLs and genes related to cucumber tender peel color disclosed by the invention comprises: (1) obtaining F1 by crossing the female parent and the male parent, and constructing recombinant inbred lines RILs; (2) the tender peel color of each strain (3) extract each RIL strain and its parental genomic DNA; (4) carry out whole genome resequencing to the parental and RIL strain genomic DNA, and carry out SNP variation detection and genotyping analysis; ( 5) Determine the LOD value threshold of each phenotype and its corresponding QTL segment; (6) Compare the SNP detection results in the QTL segment to the cucumber reference genome, search for related genes, and perform gene function annotation. The advantage of the present invention is that the method uses a high-throughput sequencing method to identify the genotypes of self-created high-generation RILs, and obtains the number of molecular markers and high-density linkage maps that cannot be achieved by traditional methods. Genes provide support.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a method for identifying QTL and genes related to cucumber tender peel color. Background technique [0002] Cucumber is one of the main vegetables cultivated in open fields and facilities in my country. Because of its delicate flavor, crisp taste and rich nutrition, it is deeply loved by consumers. Cucumber uses the tender fruit as an edible organ, and it is suitable for fried food or raw food in cold salad. Therefore, the color of its commercial fruit is not only the main factor to attract consumers, but also the main target traits that breeders focus on researching and improving. [0003] Many previous studies on fruit color of horticultural crops have shown that peel color is a quantitative trait, and the inheritance of fruit color will show different dominance and recessiveness with different crops and their hybrid combinations. Due to the different con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 李锡香沈镝窦文文董洪霞邱杨王海平宋江萍张晓辉麦尔旦·努尔阿伍提
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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