Method for simultaneously locating two character-related genes

Abstract

The invention belongs to the technical field of molecular genetics and molecular breeding, and discloses a method for simultaneously locating two character-related gene loci by using recombination phenotype grouping analysis. The method, based on the analysis of a genome re-sequenced or transcriptome sequenced recombination phenotype individual group, simultaneously locates two independent character-related gene loci, specifically comprises: constructing a separate population by using two parents with different characters; selecting, from the population, two recombination phenotype individualswith character phenotypes of different parental types; classifying the two recombination phenotype individuals into two types: one of which has a character A of a maternal type and a character B of apaternal type, and the other one of which has a character A of a paternal type and a character B of a paternal type; re-sequencing or transcriptome sequencing a mixed pool of parents and the two individual groups or the genomes of the individuals to calculate a difference of the SNP frequencies of a parental type corresponding to the SNPs having different parents in the individual group, and simultaneously locating two character-related gene loci.

Description

Technical field: [0001] The invention belongs to the technical field of molecular genetics and molecular breeding, and relates to a method for simultaneously locating two trait-related gene loci, based on genome resequencing or transcriptome sequencing technology, using a single grouping analysis of two phenotype recombined individuals for simultaneous positioning Two independent trait-related genes improve the mapping efficiency. Background technique: [0002] Bulked Segregant Analysis (Bulked Segregant Analysis, BSA), also known as separate individual grouping mixing analysis or mixed grouping analysis, was proposed for the first time by R.W.Michelmore et al. (PNAS.1991.88 (21): 9828) and successfully screened out and A marker linked to a disease resistance gene. This method first selects a certain number of plants from any segregated population produced by a pair of parents with phenotypic differences of the target trait, respectively, to form subpopulations or groups of...

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Problems solved by technology

The BSA method overcomes the limitation that it is difficult to obtain near-isogenic lines for many crops, and saves time and effort compared with the near-isogenic line method. It is a very practical method for locating genes related to target traits and is widely used; this method has many advantages, but There is a limitation, that is, a single two mixed pool analysis only locates the relevant loci for one target trait, while the genetic linkage mapping of the whole population can simultaneously locate and analyze multiple traits on the basis of genetic map construction. However, the construction of the genetic map of the whole population requires the analysis of molecular marker data of a large number of isolated populations, and the workload is relatively large; there is an urgent need for a method that can realize the genetic mapping of two traits at the same time on the basis of less workload

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