Lotus root InDel molecular marker and development method and application thereof

A molecular marker and lotus root technology, applied in recombinant DNA technology, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of high success rate of polymorphic markers, achieve low cost, improve work efficiency, and high efficiency

Active Publication Date: 2017-03-29
SHANDONG RICE RES INST
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Problems solved by technology

With the development of genomics and bioinformatics, a large number of public data such as EST, cDNA and genome sequences have emerged, making it possible to obtain candidate InDels through bioinformatics methods. A significant advantage of this type of marker is that it is based on multiple The success rate of InDel polymorphic markers ...

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  • Lotus root InDel molecular marker and development method and application thereof
  • Lotus root InDel molecular marker and development method and application thereof
  • Lotus root InDel molecular marker and development method and application thereof

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Embodiment Construction

[0024] In order to make the object, technical solution and effect of the present invention more clear and definite, the present invention will be further described in detail below. It should be understood that the specific implementation examples described here are only used to explain the present invention, and are not intended to limit the present invention.

[0025] In order to improve the success rate of lotus root InDel marker development, the present invention re-sequenced the genomes of the lotus variety Qitou and the sub-lotus variety Space 36 in the main categories of lotus roots, and then compared it with the reference genome Chinese ancient lotus genome, and screened all three. Differential InDel sites for marker development.

[0026] (1) Resequencing and data analysis of lotus root samples

[0027] After the genomic DNA of the sample is qualified, the DNA is fragmented by mechanical fragmentation (ultrasonic wave), and then the fragmented DNA is purified, repaired...

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Abstract

The invention discloses a set of lotus root InDel molecular markers and a development method and application thereof. According to the development method, genome resequencing data of a lotus variety, namely Qitou, and a seed lotus variety, namely space No.36, are compared with a Chinese ancient lotus seed reference genome; InDel loci different from one another among the lotus variety, namely Qitou, the seed lotus variety, namely space No.36, and the Chinese ancient lotus seed reference genome are screened; PCR primers are designed to conduct detection; and a set of 46 pairs of primers is selected according to the positions of the primers on lotus root chromosomes. The development method can be applied to the studies such as construction of a genetic map, QTL location, molecular assisted breeding and genetic diversity analysis of a lotus root. The InDel polymorphic markers developed by the development method have the advantages of being high in success rate, flexible in detection, simple in operation, low in cost and the like.

Description

technical field [0001] The invention relates to the technical field of molecular marker technology, in particular to a group of lotus root InDel molecular markers, a development method and application thereof. Background technique [0002] Lotus root (Nelumbo nucifera Geartn) is a perennial aquatic herbaceous plant of the genus Nelumbo nucifera, and is the aquatic vegetable with the largest cultivated area in my country. According to different production purposes, lotus can be divided into Hualien, Zilian and Oulian. The national cultivation areas of Oulian and Zilian are about 6 million mu and 1.5 million mu respectively. [0003] At present, DNA molecular markers have been widely used in various fields such as germplasm resource research, genetic map construction, target gene mapping and molecular marker-assisted selection. The development of lotus root molecular markers is mainly divided into two stages: before the lotus genome sequencing is completed, the initial low-th...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 吴修律文堂李效尊尹静静徐国鑫阴筱
Owner SHANDONG RICE RES INST
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