Lotus root InDel molecular marker and development method and application thereof

Abstract

The invention discloses a set of lotus root InDel molecular markers and a development method and application thereof. According to the development method, genome resequencing data of a lotus variety, namely Qitou, and a seed lotus variety, namely space No.36, are compared with a Chinese ancient lotus seed reference genome; InDel loci different from one another among the lotus variety, namely Qitou, the seed lotus variety, namely space No.36, and the Chinese ancient lotus seed reference genome are screened; PCR primers are designed to conduct detection; and a set of 46 pairs of primers is selected according to the positions of the primers on lotus root chromosomes. The development method can be applied to the studies such as construction of a genetic map, QTL location, molecular assisted breeding and genetic diversity analysis of a lotus root. The InDel polymorphic markers developed by the development method have the advantages of being high in success rate, flexible in detection, simple in operation, low in cost and the like.

Description

technical field [0001] The invention relates to the technical field of molecular marker technology, in particular to a group of lotus root InDel molecular markers, a development method and application thereof. Background technique [0002] Lotus root (Nelumbo nucifera Geartn) is a perennial aquatic herbaceous plant of the genus Nelumbo nucifera, and is the aquatic vegetable with the largest cultivated area in my country. According to different production purposes, lotus can be divided into Hualien, Zilian and Oulian. The national cultivation areas of Oulian and Zilian are about 6 million mu and 1.5 million mu respectively. [0003] At present, DNA molecular markers have been widely used in various fields such as germplasm resource research, genetic map construction, target gene mapping and molecular marker-assisted selection. The development of lotus root molecular markers is mainly divided into two stages: before the lotus genome sequencing is completed, the initial low-th...

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Problems solved by technology

With the development of genomics and bioinformatics, a large number of public data such as EST, cDNA and genome sequences have emerged, making it possible to obtain candidate InDels through bioinformatics methods. A significant advantage of this type of marker is that it is based on multiple The success rate of InDel polymorphic markers among varieties developed by the DNA sequence information of each variety is high, and the polymorphic primer screening process similar to SSR markers is not required, and the work efficiency is significantly improved. It has been obtained in major crops such as rice, corn, cucumber, and cabbage. Widely used, but not yet reported in lotus root

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