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Kit for guiding human mental disease medication and detection method thereof

A technology for mental illness and detection methods, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems of lack of genetic testing and medication guidance products, high testing cost, and long time period.

Active Publication Date: 2019-05-21
SHANGHAI CONLIGHT MEDICAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kit for guiding the medication of human mental illness and its detection method, so as to solve the problem of high detection cost due to the high accuracy of whole genome resequencing and whole exome sequencing technologies in the prior art. Higher, the time period is longer, so there is still a lack of appropriate products for genetic testing and medication guidance for mental diseases

Method used

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  • Kit for guiding human mental disease medication and detection method thereof
  • Kit for guiding human mental disease medication and detection method thereof
  • Kit for guiding human mental disease medication and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 Detailed description of the relevant kit

[0059] 1.1 Inspection principle

[0060] The detection method adopted in the present invention is multiplex PCR combined with nucleic acid flight mass spectrometry. First, 16 target sequences are simultaneously amplified in one system by multiplex PCR, and then specific extension primers for each SNP sequence are added to extend 1 base at the SNP site. Then, the sample analyte prepared above is co-crystallized with the chip matrix and excited by a strong laser in the vacuum tube of the mass spectrometer, and the nucleic acid molecules are desorbed into singly charged ions. The ion flight time in the electric field is inversely proportional to the ion mass. The precise molecular weight of the sample analyte can be obtained through the time-of-flight in the sample, so as to detect the SNP site information.

[0061] 1.2 Main components

[0062] The main components of the kit include: 10XPCR buffer, MgCl 2 Solution...

Embodiment 2

[0119] Example 2 Comparison of the typing effects of different primer combinations on samples

[0120] Using the same 15 human-derived DNA templates as samples, the kit A, kit B, and comparison primer combinations C and D provided by the present invention were used to genotype these 15 samples, and the results were as follows:

[0121] 2.1 The typing effect of the kit A provided by the invention

[0122] According to a kit A for guiding the medication of human mental diseases provided by the present invention, 16 gene loci can be typed at the same time, and the kit includes 16 pairs of amplification primers SEQ ID for amplifying 16 gene fragments No.1-SEQ ID No.32, and 16 extension primers SEQ ID No.33-SEQ ID No.48, the specific sequences are shown in the sequence list.

[0123] After experimental verification, in the use of this kit A, preferably, the molar concentration of 16 pairs of amplification primers in the amplification primer mixture is equivalent, that is, the amou...

Embodiment 3

[0154] The experimental example of embodiment 3 kit A

[0155] Experimental example 1: The test kit obtained the following results:

[0156]

[0157] Based on the above results, we make the following interpretations:

[0158]

[0159]

[0160] Therefore, we can make recommendations for the use of the following drugs, as shown in the table below:

[0161]

[0162] Experiment example 2:

[0163] The test kit obtained the following results:

[0164]

[0165]

[0166] Based on the above results, we make the following interpretations:

[0167]

[0168] Therefore, we can make recommendations for the use of the following drugs, as shown in the table below:

[0169]

[0170]

[0171] Experiment example 3:

[0172] The test kit obtained the following results:

[0173]

[0174] Based on the above results, we make the following interpretations:

[0175]

[0176]

[0177] Therefore, we can make recommendations for the use of the following drugs, as...

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Abstract

The invention provides a kit for guiding human mental disease medication and a detection method thereof. The kit can simultaneously type 16 gene loci through one reaction, and comprises 16 pairs of amplification primers for amplifying 16 gene segments; the sequences of 16 pairs of the amplification primers are specifically SEQ ID No.1-SEQ ID No.32; the kit also comprises 16 extension primers, andthe sequences of the 16 extension primers are specifically SEQ ID No.33-SEQ ID No.48. According to the kit and the detection method thereof, through typing based on multiple PCRs and a mass spectrum sequencing technology, human genome mutation related to mental disease medication can be accurately detected; relative to whole genome re-sequencing and whole-exome sequencing, and through the kit andthe detection method of the kit, the experiment cost is also greatly reduced, the period is shortened, and therefore the kit and the detection method of the kit have the all-important practical application value.

Description

technical field [0001] The invention relates to the field of reagent kits, in particular to a reagent kit for guiding the medication of human mental diseases and a detection method thereof. Background technique [0002] Mental illness has become a high incidence disease in our country. According to statistics, more than 17% of adults in our country suffer from depression, schizophrenia and other mental illnesses. Drug treatment is currently the main clinical method for mental illness, but it is still in the stage of trial and error, and drug efficacy and adverse reactions also show significant individual differences. Among patients with schizophrenia, only 33% to 50% of patients can obtain complete remission of clinical symptoms under sufficient anti-schizophrenia drug treatment. In addition to the traditional pathology, physiology, gender, age, height, weight, compliance, etc., genetic factors are important factors that affect differences in drug responses. In recent year...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6872C12N15/11
CPCC12Q1/6872C12Q1/6883C12Q2600/156
Inventor 何熲石忆湘魏宁黄成琛何宗顺顾孝平
Owner SHANGHAI CONLIGHT MEDICAL LTD
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