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Method for rapidly discovering phenotype related gene based on probabilistic framework and resequencing technology

A genome resequencing and resequencing technology, applied in biochemical equipment and methods, microbial measurement/inspection, instruments, etc., can solve problems such as difficulty in obtaining

Active Publication Date: 2016-03-16
ZHEJIANG UNIV
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Problems solved by technology

However, it is often difficult to obtain samples with strictly the same phenotype

Method used

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  • Method for rapidly discovering phenotype related gene based on probabilistic framework and resequencing technology
  • Method for rapidly discovering phenotype related gene based on probabilistic framework and resequencing technology
  • Method for rapidly discovering phenotype related gene based on probabilistic framework and resequencing technology

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Embodiment Construction

[0083] The invention discloses a method for quickly discovering phenotype-related genes based on a probability framework and resequencing technology, models the forward genetics research process based on genome resequencing technology, and evaluates each step in the research process by calculating four indicators The impact of the design on the overall effectiveness of the research, thereby guiding the optimization of the current experimental protocol and analysis methods, to use as few samples as possible, and quickly discover genes that may be related to specific phenotypes.

[0084] Specific phenotypes refer to Mendelian trait phenotypes or quantitative trait phenotypes with obvious main effect genes. The occurrence of this type of phenotype is related to a single genotype, a single gene change or several gene changes at the same time, and this patent can be applied. Quantitative trait phenotypes without obvious major genes are not controlled by a single genotype, and this p...

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Abstract

The present invention discloses a method for rapidly discovering a phenotype related gene based on a probabilistic framework and a resequencing technology, and establishes a method based on the probabilistic framework. The method comprises: modeling a forward genetics study process based on a genome resequencing technology, estimating, by calculating four indexes, an effect of a design of each step in a study process on overall availability of the study, thereby guiding optimization of a current experimental scheme and analytical method, and achieving the purpose of rapidly discovering a gene possibly associated with a particular phenotype by using samples as few as possible. According to the method provided by the present invention, the estimation, according to four indexes, of the overall availability of the forward genetics study process based on the genome resequencing technology is firstly proposed, and a method success rate and a non-Mendelian phenotype significance are two indexes proposed creatively, and have a significant value on guiding the optimization of an overall study process.

Description

technical field [0001] The present invention can quickly discover phenotype-related genes by sequencing the genomes of several independent individuals with the same phenotype, specifically, it is a method for quickly discovering phenotype-related genes based on a probability framework and resequencing technology. Background technique [0002] Sequencing technology is developing rapidly, and the cost is continuously decreasing while the accuracy is continuously improving. The currently popular sequencing technology can complete the whole genome resequencing of a human individual within one month, and the base accuracy is more than 99.9%. The level that the research topic group can often use. [0003] However, the massive generation of genomic data has not brought about the expected explosive improvement in biology, medicine, pharmacy, and agronomy. The main reason is that we still lack the knowledge of the relationship between genes and phenotypes, and cannot apply genomic d...

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Application Information

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IPC IPC(8): G06F19/12G06F19/18C12Q1/68
Inventor 陈新朱忠旭王纬韬
Owner ZHEJIANG UNIV
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