The invention discloses a preparation method of a vegetable seedling raising matrix, which comprises the following steps of preparing materials including seaweed meal, cow dung, pig manure, straw powder, maize cob smashed materials, perlite, vermiculite, plant ash, ephedra oil, cinnamon, honeylocust, galangal, sodium alginate, potassium fulvic, rhodofix, sodium carboxymethyl starch, a sodium chloride aqueous solution (mass percentage: 12%) and fine soil; A, fermenting raw materials: after the seaweed meal, the cow dung, the pig manure, the straw powder, the maize cob smashed materials and the fine soil are uniformly mixed, controlling the moisture content at 57-62%, and adding a corrosion promoter; B, preparing a traditional Chinese medicine extracting solution; C, taking the plant ash, and obtaining a plant ash leaching solution; D, adding the sodium alginate, the potassium fulvic, the rhodofix and the sodium carboxymethyl starch into the sodium chloride aqueous solution (mass percentage: 12%); E, soaking the vermiculite in the traditional Chinese medicine extracting solution; and F, proportioning the fermented material obtained in the step A, the perlite obtained by soaking in the step D and the vermiculite obtained by soaking in the step E in a ratio of 8:1:1. The method disclosed by the invention is simple in proportioning, easy to use and good in seedling raising effect; and seedling root systems are developed, good in water-retaining property, and rich in nutritional ingredients.