Production method of cryptocryon irritans-resistant acanthopagrus schlegelii fries

A technology to stimulate Cryptocaryon and Cryptocaryon black bream, applied in the production of disease-resistant fry and anti-stimulatory Cryptocaryon fry, can solve the problem that the effective immune protection period can only reach 3 months, and there is no detailed research on seawater fish The impact of acquired immune protection and other issues, to achieve the effect of long effective immune protection, simple and easy operation, and reduced mortality

Inactive Publication Date: 2016-08-24
SOUTH CHINA AGRI UNIV +1
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Problems solved by technology

In recent years, researchers have carried out some basic research and applied research on the immune control of Cryptocaryon stimuli, and found that the larvae of Cryptocaryon stimuli can be used to infect seawater fish such as oblique grouper and pomfret through the body surface, which can reach 80%. The abo...

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  • Production method of cryptocryon irritans-resistant acanthopagrus schlegelii fries
  • Production method of cryptocryon irritans-resistant acanthopagrus schlegelii fries

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Embodiment Construction

[0026] 1. Stimulation of live Cryptocaryon larvae collection and concentration calculation

[0027] Use a special Cryptocaryon stimuli subculture system to subculture and collect Cryptocaryon stimuli. The subculture host is Pomfret ovata. Collect well-developed Cryptocaryon stimuli cysts and place them in a sterilized clean beaker for incubation. The hatching water is high-pressure sterilized natural seawater with an appropriate amount of penicillin and streptomycin added; collect the stimulated Cryptocaryon larvae liquid within 2 hours of hatching, mix thoroughly, take 50 μL of the larvae, and add 10 μL of formaldehyde to kill the larvae. Count under a microscope, and record the live worm concentration in the larva liquid.

[0028] 2. Domestication and infection of black sea bream seedlings

[0029]Purchase about 9g of black sea bream fry, and test the fry for domestication 2 weeks before infection until they eat and move normally. During the whole breeding process, the bree...

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Abstract

The invention relates to a production method of cryptocryon irritans-resistant acanthopagrus schlegelii fries, belonging to the field of disease-resistant fry production, in particular to the field of cryptocryon irritans-resistant fry production. The method comprises the steps of firstly, producing disease-resistant acanthopagrus schlegelii fries by a variable temperature infection method and a variable temperature aquaculture method, maintaining the water temperature during infection to have a certain difference with the aquaculture water temperature within 10 days after infection so as to enhance an immune effect for live larvae of cryptocryon irritans, and further combining with rotational culture to obtain the cryptocryon irritans-resistant acanthopagrus schlegelii fries with a longer effective immune protection period. The acanthopagrus schlegelii fries also have 70 percent of relative percent survival at the eighth month after immunity, and still have 42 percent of relative percent survival at the ninth month after immunity.

Description

technical field [0001] The technology relates to a method for producing anti-stimulant Cryptocaryonia black sea bream fry, which belongs to the field of disease-resistant fry production, specifically the field of anti-stimulant Cryptocaryon fry production. Background technique [0002] Black sea bream (Acanthopagrus schlegelii), belonging to the genus Pryridae and genus, is a coastal fish and generally does not migrate long distances. It is an excellent species for breeding and releasing in China's coastal areas, and it is also one of the rare species for mariculture in southeast China. [0003] Cryptocaryon irritans, commonly known as marine fish white spot disease, is a deadly parasitic disease caused by an obligate parasitic ciliate of marine fish——Cryptocaryon irritans (Colorni and Burgess, 1997) . In recent years, Cryptocaryoniasis has become one of the most serious parasitic diseases in my country, causing great harm to seawater fishery (Luo et al., 2008), and is list...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K67/033
CPCA01K61/00A01K67/033Y02A40/81
Inventor 但学明李安兴李言伟莫泽权
Owner SOUTH CHINA AGRI UNIV
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