Production method of cryptocryon irritans-resistant acanthopagrus schlegelii fries
A technology to stimulate Cryptocaryon and Cryptocaryon black bream, applied in the production of disease-resistant fry and anti-stimulatory Cryptocaryon fry, can solve the problem that the effective immune protection period can only reach 3 months, and there is no detailed research on seawater fish The impact of acquired immune protection and other issues, to achieve the effect of long effective immune protection, simple and easy operation, and reduced mortality
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[0026] 1. Stimulation of live Cryptocaryon larvae collection and concentration calculation
[0027] Use a special Cryptocaryon stimuli subculture system to subculture and collect Cryptocaryon stimuli. The subculture host is Pomfret ovata. Collect well-developed Cryptocaryon stimuli cysts and place them in a sterilized clean beaker for incubation. The hatching water is high-pressure sterilized natural seawater with an appropriate amount of penicillin and streptomycin added; collect the stimulated Cryptocaryon larvae liquid within 2 hours of hatching, mix thoroughly, take 50 μL of the larvae, and add 10 μL of formaldehyde to kill the larvae. Count under a microscope, and record the live worm concentration in the larva liquid.
[0028] 2. Domestication and infection of black sea bream seedlings
[0029]Purchase about 9g of black sea bream fry, and test the fry for domestication 2 weeks before infection until they eat and move normally. During the whole breeding process, the bree...
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