Molecular biology method of identifying black sea bream

Abstract

The invention relates to a molecular biology method of identifying a black sea bream. The method mainly comprises the steps of designing a universal primer pair of perciformes fishes; conducting amplification sequence detection on deoxyribonucleic acid (DNA) samples of common valuable and rare perciformes fishes through the universal primer pair, selecting a particular DNA section of each kind of common valuable and rare perciformes fish according to sequence detection results, selecting a specific primer of the black sea bream from the DNA section, matching one primer in the universal primer pair with the specific primer to constitute a specific primer pair of the black sea bream, using the specific primer pair to conduct amplification on the samples of the common valuable and rare perciformes fishes, obtaining an identification standard electrophoretic band of the black sea bream according to an electrophoretic band of products, mixing the universal primer pair and the specific premier of the black sea bream so as to form a mixing premier, conducting amplification on DNA samples of fishes to be identified by adoption of the mixing premier, and comparing the electrophoretic band of the products and the identification standard electrophoretic band so as to obtain a species identification result.

Description

technical field [0001] The invention relates to a method for identifying common and rare perciformes, in particular to a molecular biology method for identifying black sea bream. Background technique [0002] To protect biodiversity is to protect human beings. Due to the particularity of the water ecological environment and the fact that most aquatic animals have been caught by people, fish biodiversity is more vulnerable than terrestrial animal diversity and is under greater pressure. To protect fish germplasm resources is to protect the production source of aquatic animal protein necessary for human survival. Fish resource is a renewable resource. As long as it is used properly, it can be inexhaustible and enduring for a long time. However, if it is overfished or overfished, the resource may be exhausted or the individual will become smaller and the quality will decrease. Fishing is not a negative factor. As long as fishing is reasonable, it can maximize the production o...

AI Technical Summary

Problems solved by technology

This makes identification difficult and error-prone in situations such as juvenile fish, broken fish, or decay

In recent years, many laboratories at home and abroad have begun to use mitochondrial DNA typing, restriction length polymorphism, isoenzyme electrophoresis, and random primer amplification polymorphism (RAPD) techniques to identify fish, but these methods are generally cumbersome and time-consuming. , laborious, expensive, not conducive to operation, poor repeatability, large errors and other disadvantages