Biosynthesis method for temperature-resistant dendritic slow-digestion starch

A slow-digesting starch and biosynthesis technology, applied in food science, pharmaceutical formulation, drug delivery, etc., can solve problems such as rare research, and achieve the effect of simple process, wide source of raw materials, and high nutritional value

Active Publication Date: 2010-10-06
SHANDONG ZHONGGU STARCH SUGAR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research institutions of SDS are mainly concentrated in European and American countries, and the relevant repo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 1g of waxy corn starch to make a starch milk solution with a concentration of 20%, and then cook it at 80°C for 30 minutes to make it completely gelatinized; when the starch paste is cooled to room temperature, add 26mL of sodium acetate buffer solution with a pH of 5.5 A new type of amylase mixed solution with 1000U / g substrate, wherein the mixed enzyme solution is composed of β-amylase and transglucosidase (volume ratio 1:1); keep stirring and constant temperature reaction at 55°C for 10h; deenzyme treatment Finally, the membrane ultrafiltration treatment with a molecular weight cut-off of 650Da is used, and then the obtained product is dried and crushed to obtain a temperature-resistant dendritic slow-digesting starch. The SDS content is 78% and the branch density is 35%. After high-temperature cooking, the SDS content is basically No change.

Embodiment 2

[0027] Weigh 1g of potato starch to make a starch milk solution with a concentration of 1%, and then cook it at 100°C for 5min to make it completely gelatinized; when the starch paste is cooled to room temperature, add 10mL of sodium acetate buffer solution with pH 4.5 and A new type of amylase mixed solution per gram of substrate, wherein the mixed enzyme solution is composed of branched amylase and maltosyl amylase (volume ratio 1:10); kept stirring at 35°C for 24 hours at a constant temperature; The membrane ultrafiltration treatment with a molecular weight cut-off of 500 Da is adopted, and then the obtained product is dried and pulverized to obtain the temperature-resistant dendritic slow-digesting starch. The SDS content was determined to be 65%, the branch density of the temperature-resistant dendritic slow-digesting starch was 19%, and the content of the temperature-resistant dendritic slow-digesting starch was basically unchanged after high-temperature cooking.

Embodiment 3

[0029] Weigh 1g of indica rice starch to make a starch milk solution with a concentration of 40%, and then cook it at 90°C for 15 minutes to make it completely gelatinized; A new type of amylase mixed solution of g substrate, wherein the mixed enzyme solution is composed of maltosyl amylase and maltose transglucosylase (volume ratio 1:90); constant stirring and constant temperature reaction at 90°C for 1.5h; enzyme inactivation treatment The final sample liquid is processed by membrane ultrafiltration with a molecular weight cut-off of 1000 Da, and then the obtained product is dried and pulverized to obtain the temperature-resistant dendritic slow-digesting starch. The SDS content was determined to be 71%, the branch density of the temperature-resistant dendritic slow-digesting starch was 24%, and the content of the temperature-resistant dendritic slow-digesting starch was basically unchanged after high-temperature cooking.

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Abstract

The invention provides a biosynthesis method for temperature-resistant dendritic slow-digestion starch, which belongs to the technical field of non-chemically modified starch. The method utilizes a biological catalytic conversion technique for beta-amylase, amylomaltase, maltose-base amylase, chorismic amylase and transglucosylase to recombine starch molecular chain of different-source commercialized starch through synergistic modification of novel hydrolysis-transglycosylation, such as corn starch, wheat starch, rice starch, potato starch, cassava starch, sweet potato starch, mung bean starch, chickpea starch, sorghum starch, sago starch, banana taro starch and the like, thereby obtaining the temperature-resistant dendritic starch with the characteristic of slow digestion. The temperature-resistant dendritic slow-digestion starch obtained by the method can be used as a functional raw/auxiliary material to develop hypoglycemia healthy foods, energy slow-release foods, nutritional factor-targeted controlled-release carrier materials and the like.

Description

technical field [0001] The invention relates to a method for synthesizing temperature-resistant dendritic slow-digesting starch from natural starch by biological enzyme method, and belongs to the technical field of non-chemically modified starch. Background technique [0002] With changes in social structure, accelerated pace of life, changes in eating habits and food composition, and aging society, the spectrum of human diseases has undergone great changes. Chronic diseases, such as diabetes, cardiovascular disease, obesity, hyperlipidemia, The increasing proportion of insulin resistance-related diseases such as hypertension has become a major global public health problem. The key to preventing the above-mentioned diseases lies in a scientific and reasonable dietary structure. [0003] Slowly digestible starch (SDS) refers to those starches that are completely digested and absorbed in the small intestine but at a slower rate. According to the classification of starch bioa...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04A23L1/29A23L1/30A61K47/36A61K9/52A23L33/125
Inventor 缪铭江波张涛沐万孟
Owner SHANDONG ZHONGGU STARCH SUGAR
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