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Extracting method of natural pigment glycosidoprotein in eucheuma

A technology of natural pigments and extraction methods, applied in peptide preparation methods, chemical instruments and methods, algae/bryopeptides, etc., can solve problems such as low stability, waste of resources, and complex extraction processes

Inactive Publication Date: 2009-10-21
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of phycobiliproteins at home and abroad mainly adopts the method of alkaline liquid extraction combined with repeated freezing and ultrasonic cell destruction, which has the defects of complicated extraction process, high cost, small product quantity, low stability and waste of resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0009] Take by weighing 10g of Eucheuma washed and cut into strips, place it in 50ml hydrochloric acid solution with a pH value of 3.0, and extract it for 36h, filter under reduced pressure with a suction filter, obtain a total of 45ml of filtrate, concentrate it to 20ml under reduced pressure with a rotary evaporator, adjust The pH value of the concentrated solution is 4.5, continue to add 40ml of 95% ethanol, stir, leave overnight, precipitate out, vacuum filter, wash the precipitated part on the filter paper for 3 times with 10ml of absolute ethanol, and then wash with 10ml of acetone Twice, dry at room temperature to obtain natural pigment glycoprotein.

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Abstract

In an extracting method of natural pigment glycosidoprotein in eucheuma, the eucheuma is disposed in hydrochloric acid solution for leaching and is filtered under reduced pressure by a suction filter machine to obtain filter liquor, the filter liquor is condensed under reduced pressure by a rotary evaporimeter, dilute hydrochloric acid is added for adjusting pH value, and ethanol is continuously added, precipitate is precipitated after stirring and precipitating, vacuum filtration is carried out, the precipitate on filter paper is firstly washed with absolute ethyl alcohol for three times and then with acetone for twice, and the precipitate is dried at room temperature, thereby obtaining natural pigment glycosidoprotein.

Description

Technical field: [0001] The invention relates to a method for extracting natural pigment glycoproteins in Eucheuma. The natural pigment glycoproteins are obtained by soaking, filtering, and precipitating and separating Eucheuma by acid solution extraction, which belongs to the field of active substance extraction of biotechnology. Background technique: [0002] Eucheuma grows in tropical and subtropical red algae seaweed, and its soluble seaweed polysaccharide content is extremely rich, which is the main raw material for extracting carrageenan at present. The surface tissue of Eucheuma contains a phycobiliprotein, which has the functions of photosynthesis and energy generation, conversion and transmission, and has broad applications in biomedical fields such as fluorescence immunological clinical diagnosis in vivo and photodynamic therapy of tumors prospect. At present, the extraction of phycobiliproteins at home and abroad mainly adopts the method of alkaline liquid extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/14
Inventor 全香花钟进义
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV