Extracting method of natural pigment glycosidoprotein in eucheuma

Abstract

In an extracting method of natural pigment glycosidoprotein in eucheuma, the eucheuma is disposed in hydrochloric acid solution for leaching and is filtered under reduced pressure by a suction filter machine to obtain filter liquor, the filter liquor is condensed under reduced pressure by a rotary evaporimeter, dilute hydrochloric acid is added for adjusting pH value, and ethanol is continuously added, precipitate is precipitated after stirring and precipitating, vacuum filtration is carried out, the precipitate on filter paper is firstly washed with absolute ethyl alcohol for three times and then with acetone for twice, and the precipitate is dried at room temperature, thereby obtaining natural pigment glycosidoprotein.

Description

Technical field: [0001] The invention relates to a method for extracting natural pigment glycoproteins in Eucheuma. The natural pigment glycoproteins are obtained by soaking, filtering, and precipitating and separating Eucheuma by acid solution extraction, which belongs to the field of active substance extraction of biotechnology. Background technique: [0002] Eucheuma grows in tropical and subtropical red algae seaweed, and its soluble seaweed polysaccharide content is extremely rich, which is the main raw material for extracting carrageenan at present. The surface tissue of Eucheuma contains a phycobiliprotein, which has the functions of photosynthesis and energy generation, conversion and transmission, and has broad applications in biomedical fields such as fluorescence immunological clinical diagnosis in vivo and photodynamic therapy of tumors prospect. At present, the extraction of phycobiliproteins at home and abroad mainly adopts the method of alkaline liquid extrac...

AI Technical Summary

Problems solved by technology

At present, the extraction of phycobiliproteins at home and abroad mainly adopts the method of alkaline liquid extraction combined with repeated freezing and ultrasonic cell destruction, which has the defects of complicated extraction process, high cost, small product quantity, low stability and waste of resources.