Skin grafting material and use method thereof

Abstract

The invention relates to a skin grafting material and a using method thereof. The skin grafting material is composed of compressed sponge and repairing liquid. The compressed sponge is selected from chitosan sponge, collagen sponge or polyvinyl formal sponge; the repairing liquid is selected from normal saline containing human epidermal growth factors and / or normal saline containing superoxide dismutase. The use method comprises the following steps: attaching the skin layer of the cut skin graft to the compressed sponge, then putting the compressed sponge into a medical skin rolling machine, and cutting the skin graft into micro skin graft; the cut miniature skin graft is soaked in the repairing liquid, is attached to the position to be subjected to skin grafting after fully absorbing the liquid and expanding, and is wrapped with gauze. The skin grafting material provided by the invention can reduce damage to newborn granulation when the skin grafting material is removed, has an antibacterial effect and improves scar hyperplasia contracture after a skin grafting wound operation; meanwhile, the sponge which absorbs the liquid and expands can slowly release the repairing liquid, so that skin cell regeneration is promoted, epithelization of a skin grafting area is accelerated, and wound healing is promoted; the healing effect is good, and the application prospect is wide.

Description

Technical Field

[0001] The invention belongs to the field of biomedical materials, and in particular, relates to a skin grafting material and a use method thereof. Background Art

[0002] At present, the skin grafting methods for repairing large-area burn wounds in clinical practice mainly include autologous microskin combined with allogeneic large skin transplantation, micro-skin grafting (MEEK) and traditional stamp skin grafting. Stamp skin grafting can expand the skin grafting area to a certain extent, but it is difficult to treat patients with a burn area of ​​more than 90%; autologous microskin combined with allogeneic large skin transplantation can significantly improve the success rate of treatment, but the probability of scar hyperplasia and contracture after transplantation is high, and the long-term effect cannot be guaranteed. In addition, it is difficult to obtain allogeneic skin sources, which limits the development of this technology. MEEK skin grafting technology can effectively increase the proportion of autologous skin expansion and has a high survival rate. Depending on the expansion ratio, epithelialization is completed in about 10 days after surgery. At present, conventional MEEK skin grafting materials are composed of cork pads and aluminum foil-polyamide double crepe yarn. After the autologous skin is cut and adhered to the double pleated yarn, it is applied to the wound and then bandaged with multiple layers of gauze. The polyamide double crepe yarn used in the prior art cannot prevent the invasion of foreign bacteria during the wound repair process, and there is a risk of damaging new granulation tissue when removing the material.

[0003] In view of this, it is particularly necessary to invent a skin grafting material that can promote wound healing, has antibacterial effects, and can reduce damage to new granulation tissue when removed, without affecting the effect of skin grafting, so as to improve scar hyperplasia and contracture after transplantation at the wound and accelerate epithelialization of the skin grafting area. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a skin graft material which can promote wound healing, keep the wound moist, thereby accelerating the epithelialization of the skin graft area, and at the same time improve scar hyperplasia and contracture after transplantation at the wound.

[0005] To solve the above problems, the technical solution adopted by the present invention is: Technical topic 1: The present invention provides a skin grafting material, which is composed of a compressed sponge and a repairing liquid; The compressed sponge is selected from chitosan sponge, collagen sponge or polyvinyl formal sponge; The repair solution is selected from physiological saline containing human epidermal growth factor and / or physiological saline containing superoxide dismutase.

[0006] As a further embodiment of the present invention, the repair solution is physiological saline containing human epidermal growth factor and superoxide dismutase; the content of the human epidermal growth factor is 100-200 U / mL, and the content of the superoxide dismutase is 500-1000 U / mL.

[0007] As a further embodiment of the present invention, the total titer of human epidermal growth factor and superoxide dismutase in the physiological saline containing human epidermal growth factor and superoxide dismutase is 600-1200 U / mL.

[0008] As a further embodiment of the present invention, the repair fluid is a physiological saline containing human epidermal growth factor, and the content of human epidermal growth factor in the physiological saline containing human epidermal growth factor is 200-400 U / mL.

[0009] As a further embodiment of the present invention, the repair solution is a physiological saline containing superoxide dismutase, and the content of superoxide dismutase in the physiological saline containing superoxide dismutase is 1000-2000 U / mL.

[0010] As a further embodiment of the present invention, the area expansion ratio of the compressed sponge contacting the leather sheet surface is 1:2-4.

[0011] As a further embodiment of the present invention, the water absorption rate of the compressed sponge is 10-15 times.

[0012] As a further embodiment of the present invention, the water absorption rate of the compressed sponge is 10-12 times.

[0013] Technical theme 2: The present invention also provides a method for using the skin grafting material as described in the first technical theme, the method comprising: attaching the epidermal layer of the cut skin piece to a compressed sponge, and then placing the sponge into a medical skin rolling machine to cut it into micro skin pieces; soaking the cut micro skin pieces in a repair liquid, and attaching the compressed sponge with the skin piece to the area to be grafted after it has fully absorbed the liquid and expanded, and then wrapping it with gauze.

[0014] As a further embodiment of the present invention, the micro-skin graft has a length×width of 2-5 mm×2-5 mm.

[0015] The beneficial effects of adopting the above technical solution are: 1. The skin graft material provided by the present invention is composed of a compressed sponge and a repair fluid, wherein the compressed sponge is selected from a chitosan sponge, a collagen sponge or a polyvinyl alcohol formal sponge. The chitosan sponge and collagen can be absorbed by the human body to promote wound healing; even if the material is not completely absorbed, the compressed sponge soaked in the repair fluid is in a gel state and is easy to remove, which can greatly reduce the damage to the wound when removing the skin graft material.

[0016] 2. The repair solution is selected from physiological saline containing human epidermal growth factor and / or superoxide dismutase, wherein the human epidermal growth factor and superoxide dismutase have the effects of antibacterial and anti-inflammatory, promoting wound healing and reducing postoperative scar hyperplasia and contracture, and are beneficial to the healing of the skin grafted area.

[0017] 3. Use the compressed sponge together with the repair fluid. After fully absorbing the repair fluid, use the compressed sponge on the skin grafted area to keep the wound in the repair fluid environment for a long time, reducing the invasion of foreign bacteria. The human epidermal growth factor and superoxide dismutase in the fluid are released over a long period of time, further promoting the recovery of the skin grafted area and reducing postoperative scar hyperplasia and contracture.

[0018] 4. The skin graft material provided by the present invention is simple to prepare, easy to use and transport, and at the same time, the material has high stability, has a good growth-promoting effect on the affected area, and has broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 Schematic diagram of the compressed sponge used in Example 1 of the present invention, wherein (a) is before compression and (b) is after compression; Figure 2 This is a schematic diagram of the structure of the compressed sponge with a leather patch after cutting in Example 1 of the present invention; Figure 3 It is a schematic diagram of the structure of the compressed sponge with a micro-skin patch after being fully immersed in the repair liquid according to Example 1 of the present invention. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical scheme and advantages of the present invention clearer, the invention is described clearly and completely in conjunction with specific embodiments below. Unless otherwise specified, the equipment used in this embodiment is conventional equipment, the reagents used are conventional reagents, all of which can be purchased commercially, and the operating methods used are technical means known to those skilled in the art.

[0021] It should be noted that the chitosan sponge used in the embodiment of the present invention was purchased from Shijiazhuang Yishengtang Medical Products Co., Ltd.; the collagen sponge was purchased from Hebei Corey Biotechnology Co., Ltd.; the polyvinyl formal sponge was purchased from Dongguan Yuchuanghong Polymer Materials Co., Ltd., model PVFM1; the human epidermal growth factor was purchased from Shanghai Tengrui Pharmaceutical Co., Ltd., specification 25000U / 2ml / tube; the superoxide dismutase was purchased from Jiangsu Aoyuanqi Biomedical Technology Co., Ltd., specification 2g / bottle, activity 6000U / mg.

[0022] Example 1 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 4.8 mL of human epidermal growth factor, 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL, and the content of superoxide dismutase is 500 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0023] Example 2 (1) Place a collagen sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm into a molding machine for compression, and compress it into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 4.8 mL of human epidermal growth factor, 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL, and the content of superoxide dismutase is 500 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0024] Example 3 (1) Place a polyvinyl alcohol formal sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm into a molding machine for compression, and compress it into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 4.8 mL of human epidermal growth factor, 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL, and the content of superoxide dismutase is 500 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0025] Example 4 (1) A chitosan sponge with a length of 60 mm, a width of 60 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 4.8 mL of human epidermal growth factor, 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL, and the content of superoxide dismutase is 500 U / mL; (5) Soak the micro-skin graft sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the compressed sponge are 60 mm, 60 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:2. The sponge is attached to the area to be grafted and the grafted area is wrapped with gauze.

[0026] Example 5 (1) A chitosan sponge with a length of 84 mm, a width of 84 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 4.8 mL of human epidermal growth factor, 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL, and the content of superoxide dismutase is 500 U / mL; (5) Soak the micro-skin graft sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the compressed sponge are 84 mm, 84 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:4. The sponge is attached to the area to be grafted and the grafted area is wrapped with gauze.

[0027] Example 6 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 9.6 mL of human epidermal growth factor, 60 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 200 U / mL, and the content of superoxide dismutase is 600 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0028] Example 7 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 6 mL of human epidermal growth factor, 100 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 125 U / mL, and the content of superoxide dismutase is 1000 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0029] Example 8 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 100 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the superoxide dismutase content is 1000 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0030] Example 9 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 9.6 mL of human epidermal growth factor and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 200 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0031] Example 10 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 200 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the superoxide dismutase content is 2000 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0032] Embodiment 11 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 19.2 mL of human epidermal growth factor and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 400 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0033] Comparative Example 1 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the superoxide dismutase content is 500 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0034] Comparative Example 2 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 4.8 mL of human epidermal growth factor and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0035] Comparative Example 3 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 600 mL of normal saline; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0036] Comparative Example 4 (1) Prepare a cork tray material for carrying leather, the cork tray is 42 mm long and 42 mm wide, and prepare an aluminum foil-polyurethane double crepe yarn with an expansion ratio of 1:3 after folding and pressing, and the size of the contact leather piece is 42 mm long and 42 mm wide; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Soak the cork tray with physiological saline and attach the dermis of the autologous skin to the cork tray; put the cork tray with the skin graft into a medical leather rolling machine and cut it into micro-skin grafts with a length of 3 mm and a width of 3 mm; spray adhesive on the surface of the skin graft so that the surface of the skin graft contacts the polyamide double crepe yarn, remove the cork tray, and transfer all the micro-skin grafts to the polyamide double crepe yarn; (4) Take 4.8 mL of human epidermal growth factor, 50 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 100 U / mL, and the content of superoxide dismutase is 500 U / mL; (5) After the polyurethane double crepe is expanded by pulling it horizontally and vertically, the double crepe is trimmed and the aluminum foil is removed. It is soaked in physiological saline for later use and attached to the area to be grafted. The area to be grafted is wrapped with gauze.

[0037] Investigation Example 1 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 2.4 mL of human epidermal growth factor, 55 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 50 U / mL, and the content of superoxide dismutase is 550 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0038] Investigation Example 2 (1) A chitosan sponge with a length of 73 mm, a width of 73 mm, and a height of 5 mm was placed in a molding machine for compression, and compressed into a shape with a length of 42 mm, a width of 42 mm, and a height of 2.5 mm; (2) The skin graft is selected from autologous skin, and the autologous skin is cut into skin grafts with a length of 42 mm and a width of 42 mm; (3) Attach the epidermis of the cut skin piece to the compressed sponge, put it into a medical skin rolling machine, and cut it into micro skin pieces with a length of 3 mm and a width of 3 mm; (4) Take 14.4 mL of human epidermal growth factor, 30 mg of superoxide dismutase and 600 mL of normal saline, mix and stir for 30 minutes until dissolved, wherein the content of human epidermal growth factor is 300 U / mL, and the content of superoxide dismutase is 300 U / mL; (5) Soak the microskin sponge prepared in step (3) in a physiological saline repair solution containing human epidermal growth factor and superoxide dismutase at room temperature until the compressed sponge expands no longer and then is taken out. After the compressed sponge expands after absorbing the liquid, the length, width and height of the sponge are 73 mm, 73 mm and 5 mm, and the area expansion ratio of the surface in contact with the skin graft is 1:3. Stick it on the area to be grafted and wrap the grafted area with gauze.

[0039] Test Example 1 Compressed sponge water absorption test Fill the container with 600 mL of saline solution, prepare a compressed sponge with a size of 42*42*2.5, and weigh the original weight of the compressed sponge, recorded as m. 1 , put the compressed sponge into the container, take it out after it fully absorbs the liquid and expands, and weigh its weight, recorded as m 2 The water absorption rate of compressed sponge is Q = (m 2 -m 1 ) / m 1 .

[0040] Table 1 Compressed sponge water absorption test results Compressed sponge types Chitosan Collagen Polyvinyl formal Water absorption rate 10 11 11.5 Effect Example 1 The skin grafting effects of the fully soaked skin grafting materials with micro-skin grafts obtained in Examples 1-11, Comparative Examples 1-4 and Investigation Examples 1-2 were studied. Skin grafting surgery was performed using the obtained skin grafting materials, and the skin grafting effects, wound damage during removal, and scar hyperplasia and contracture after healing were observed and recorded.

[0041] Antibiotics were infused during the skin grafting period. The skin graft material was removed one week later and the wound recovery was observed. The healing process of the affected area and the scar hyperplasia and contracture after healing were recorded. The experimental results are shown in Table 2.

[0042] Among them, 0-2 means that the skin graft material does not cause damage to the skin graft when it is removed; 3-4 means that the skin graft material causes slight damage to the skin graft when it is removed; 5-7 means that the skin graft material causes damage to the skin graft when it is removed; 8-10 means that the skin graft material causes serious damage to the skin graft when it is removed; 0-2 means no infection at the skin graft site; 3-4 means slight infection at the skin graft site; 5-7 means infection at the skin graft site; 8-10 means severe infection at the skin graft site; 0-2 means that the scar at the skin graft site has no hyperplasia and contracture after healing; 3-4 means that the scar at the skin graft site has slight hyperplasia and contracture after healing; 5-7 means that the scar at the skin graft site has hyperplasia and contracture after healing; 8-10 means that the scar at the skin graft site has severe hyperplasia and contracture after healing.

[0043] Table 2 Skin grafting effect of skin grafting materials with micro-skin grafts obtained in Examples 1-11, Comparative Examples 1-4 and Investigation Examples 1-2 Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A skin graft material, characterized in that: It is composed of compressed sponge and repair fluid; The compressed sponge is selected from chitosan sponge, collagen sponge or polyvinyl formal sponge; The repair solution is selected from physiological saline containing human epidermal growth factor and / or physiological saline containing superoxide dismutase.

2. A skin graft material according to claim 1, characterized in that: The repair solution is physiological saline containing human epidermal growth factor and superoxide dismutase; the content of the human epidermal growth factor is 100-200 U / mL, and the content of the superoxide dismutase is 500-1000 U / mL.

3. The skin grafting material according to claim 2, characterized in that: The total titer content of human epidermal growth factor and superoxide dismutase in the physiological saline containing human epidermal growth factor and superoxide dismutase is 600-1200 U / mL.

4. The skin graft material according to claim 1, characterized in that: The repair solution is physiological saline containing human epidermal growth factor, and the content of human epidermal growth factor in the physiological saline containing human epidermal growth factor is 200-400 U / mL.

5. The skin grafting material according to claim 1, characterized in that: The repair solution is a physiological saline containing superoxide dismutase, and the content of superoxide dismutase in the physiological saline containing superoxide dismutase is 1000-2000U / mL.

6. The skin graft material according to claim 1, characterized in that: The area expansion ratio of the compressed sponge contacting the leather sheet surface is 1:2-4.

7. The skin graft material according to claim 1, characterized in that: The water absorption rate of the compressed sponge is 10-15 times.

8. The skin grafting material according to claim 1, characterized in that: The compressed sponge has a water absorption rate of 10-12 times.

9. A method for using the skin graft material according to any one of claims 1 to 8, characterized in that: The method of use is: attach the epidermis of the cut skin piece to the compressed sponge, then put it into a medical skin rolling machine and cut it into micro skin pieces; soak the cut micro skin pieces in the repair liquid, wait for the compressed sponge with the skin piece to fully absorb the liquid and expand, then stick it to the area to be grafted and wrap it with gauze.

10. The method of use according to claim 9, characterized in that: The micro skin graft has a length×width of 2-5 mm×2-5 mm.

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