A combined detection kit for novel coronavirus antibodies and its application

A coronavirus and joint detection technology, which is applied in the field of joint detection kits for new coronavirus antibodies, can solve the problems of limited nucleic acid detection, high laboratory standard requirements, and long detection time, and achieve high sensitivity and specificity. Convenience and low cost effect

Active Publication Date: 2022-07-05
厦门万渤生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing detection methods are based on traditional fluorescent PCR reagents. Due to the complicated operation, the possible generation of aerosols that may cause large-scale pollution (produce false positive samples), high requirements for laboratory standards, and long detection time, etc., the current nucleic acid Testing is still very limited

Method used

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  • A combined detection kit for novel coronavirus antibodies and its application
  • A combined detection kit for novel coronavirus antibodies and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0028] A combined detection kit for novel coronavirus antibodies, comprising a diluent, the novel coronavirus IgM / IgG combined test strip includes a bottom plate and sequentially connected sample pads, binding pads, reaction membranes, and water-absorbing pads arranged on the bottom plate The reaction membrane is provided with a first detection area, a second detection area and a quality control area, and the binding pad is coated with a color-coded recombinant SARS-COV-2 antigen and a color-coded mouse IgG antibody. The detection area is coated with mouse anti-human IgM monoclonal antibody, the second detection area is coated with mouse anti-human IgG monoclonal antibody, and the quality control area is coated with goat anti-mouse IgG antibody.

[0029] When in use, drop or stain the sample liquid on the sample pad, let it stand for 5-10 minutes, and observe the color development of the first detection area, the second detection area and the quality control area.

[0030] Col...

Embodiment approach 2

[0050] This embodiment is the application of the detection kit in Embodiment 1 in the detection of novel coronavirus IgG and IgM in whole blood, serum or plasma.

[0051] Further, after the sample is placed on the sample pad, the diluent is added, and the color reaction in the quality control area and the first detection area and the second detection area is observed.

Embodiment 1

[0053] like figure 1 As shown, the combined detection kit for novel coronavirus antibodies includes a test strip 1, and the test strip includes a bottom plate and a sample pad 12, a binding pad 13, a reaction membrane 14, and a water-absorbing pad 15 that are sequentially connected on the bottom plate; The reaction membrane 14 is provided with a first detection zone 141, a second detection zone 142 and a quality control zone 143, and the binding pad 13 is coated with a color-coded recombinant SARS-COV-2 antigen and a color-coded recombinant SARS-COV-2 antigen. For mouse IgG antibody, the first detection zone 141 is coated with mouse anti-human IgM monoclonal antibody, the second detection zone 142 is coated with mouse anti-human IgG monoclonal antibody, and the quality control zone 143 is coated with goat anti-mouse IgG antibody.

[0054] Recombinant SARS-COV-2N protein antigen, using genetic engineering technology to construct plasmid, containing his-tag tag, soluble expressi...

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Abstract

The invention relates to the detection of a coronavirus antibody, in particular to a combined detection kit for a novel coronavirus antibody and its application. The kit includes a diluent, a novel coronavirus IgM / IgG combined test strip, and the novel coronavirus IgM / IgG combined test strip includes a bottom plate and sequentially connected sample pads, binding pads, A reaction membrane and a water-absorbing pad; the reaction membrane is provided with a first detection area, a second detection area and a quality control area, and the binding pad is coated with a color-coded recombinant SARS-COV-2 antigen and a color-coded mouse IgG antibody, the first detection area is coated with mouse anti-human IgM monoclonal antibody, the second detection area is coated with mouse anti-human IgG monoclonal antibody, and the quality control area is coated with goat anti-mouse IgG antibody. The invention can obtain the combined determination of IgM and IgG without special equipment, has high sensitivity, and is of great significance to the detection of early infection and long-term infection.

Description

technical field [0001] The invention relates to the detection of a coronavirus antibody, in particular to a combined detection kit for a novel coronavirus antibody and its application. Background technique [0002] SARS-COV-2 is an acute respiratory infectious disease mainly caused by infection with the 2019 novel coronavirus (2019-nCoV). The main routes of transmission are direct transmission, aerosol transmission and contact transmission. Novel coronavirus pneumonia is a typical emergency disease, and the clinical needs to know the test results as soon as possible, so as to take the next preventive measures. However, most of the existing detection methods are based on traditional fluorescent PCR reagents. Due to the complex operation, the possible generation of aerosols and causing large-scale pollution (producing false positive samples), and the high requirements for laboratory standards and long detection times, the current nucleic acid The rollout of testing is still ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/569G01N33/558
CPCG01N33/6854G01N33/577G01N33/56983G01N33/558G01N2469/20G01N2333/165Y02A50/30
Inventor 范文丽
Owner 厦门万渤生物技术有限公司
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