Bacillus pyocyaneus vaccine and preparation method thereof

Abstract

The invention discloses a Pseudomonas aeruginosa vaccine and a preparation method thereof, which relate to a vaccine and a preparation method thereof. The invention solves the problems that the prior antibiotic is difficult to cure Pseudomonas aeruginosa infection, and a Pseudomonas aeruginosa vaccine with single valence can not satisfy the demand of clinical immunity. The vaccine consists of endotoxin of Pseudomonas aeruginosa P1, endotoxin of Pseudomonas aeruginosa P2, endotoxin of Pseudomonas aeruginosa P6 and toxoid of Pseudomonas aeruginosa P103 which are placed in physiological saline. The preparation method comprises the following steps: the endotoxins of Pseudomonas aeruginosaes are prepared; the toxoid of the Pseudomonas aeruginosa P103 is prepared; and the Pseudomonas aeruginosa vaccine can be obtained by mixing the endotoxins of the Pseudomonas aeruginosaes and the toxoid of the Pseudomonas aeruginosa P103. The Pseudomonas aeruginosa vaccine belongs to a polyvalent vaccine; the endotoxins of the Pseudomonas aeruginosa P1, the Pseudomonas aeruginosa P2 and the Pseudomonas aeruginosa P6 are adopted, so known type 11 Pseudomonas aeruginosa can be immunized; and the toxoid of the Pseudomonas aeruginosa P103 is added, so the immune effect of the vaccine to the Pseudomonas aeruginosaes is strengthened.

Description

technical field

[0001] The invention relates to a vaccine and a preparation method thereof. Background technique

[0002] Pseudomonas aeruginosa is an opportunistic pathogen with strong adaptability to the external environment and can survive for a long time in a humid environment. Pseudomonas aeruginosa exists in normal human skin, especially in moist parts (such as armpits, perineum, ear canal, respiratory tract and intestinal tract, etc.), and intact skin and mucous membranes can effectively resist Pseudomonas aeruginosa as a barrier However, the normal defense mechanism of the human body is reduced or damaged (such as skin and mucous membrane damage, agranulocytosis, hypoproteinemia, radiotherapy and chemotherapy of tumors, long-term application of hormones and antibiotics, burns, infant skin, umbilical cord, intestinal tract, Urinary system of the elderly) is prone to lead to Pseudomonas aeruginosa infection.

[0003] According to reports, nosocomial infections caused...