Improved method for controlling polymerized emulsion microbial pollution by using carbon dioxide detection

A technology of carbon dioxide and polymerized emulsion, which is applied in the direction of biochemical equipment and methods, measurement/inspection of microorganisms, analysis using chemical indicators, etc., can solve problems such as product loss, and achieve the effect of minimizing sampling errors

Inactive Publication Date: 2004-05-12
AIR PRODS POLYMERS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the emulsion sample is taken from the bottom of the container, the streak plate test may detect that it is not contaminated
In this case, the presence of contamination may go unnoticed and the emulsion will continue to biodegrade leading to product loss

Method used

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  • Improved method for controlling polymerized emulsion microbial pollution by using carbon dioxide detection

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: Control

[0029] Sterilized with AIRFLEX  400 (1600g) polyvinyl alcohol-stabilized vinyl acetate / ethylene polymer emulsion is placed in a 2L plastic Nalgene container, and two holes are made on the plastic lid of the container, one of which is used to insert a carbon dioxide sensor, and the other is a small diameter Many holes are opened, so that the inside of the container is communicated with the atmosphere and keeps the pressure constant. A Vaisala GMT222 direct read CO2 converter connected to the data logger and computer was used as the measurement device. The cap was then tightened and the headspace carbon dioxide concentration was measured for 3 days. The headspace carbon dioxide concentration was stable at about 400 ppm throughout the monitoring period.

[0030] No microbial growth was observed during the control process when the polymeric emulsion was streaked.

Embodiment 2

[0031] Embodiment 2: the mensuration of carbon dioxide in polluted emulsion

[0032] To determine the efficacy of carbon dioxide measurement as a detection mechanism for microbial growth, the AIRFLEX  400 (1600 g) of polyvinyl alcohol-stabilized (with some antimicrobial agent) vinyl acetate / ethylene polymer emulsions were inoculated with gluconoacetic acid bacteria liquefaction. The concentration of liquefied gluconoacetic acid bacteria in Airflex400 emulsion was determined in potato dextrose agar by dilution plate metering method, and the result was 5.4×10 4 cfu / ml. The polluted emulsion was placed in a 2L plastic Nalgene container, and the lid was treated the same as in Example 1, with a carbon dioxide sensor hole and a vent hole, and tightened with screws. Within minutes, the amount of carbon dioxide in the headspace of the vessel began to rise above the initial value (-400 ppm). After 2 days of continuous monitoring of the sample, the carbon dioxide concentration in th...

Embodiment 3

[0033] Example 3: Measurement of Carbon Dioxide After Addition of Antimicrobial Agent

[0034] To the same contaminated sample as described in Example 2, 400 ppm of dodecylguanidine hydrochloride (DGH) antimicrobial agent was added to reduce the concentration of Gluconoacetic acid bacteria liquefied.

[0035] The concentration of carbon dioxide in the headspace of the vessel was monitored in the same manner as in Examples 1 and 2. After 2 days of continuous monitoring of the sample, the carbon dioxide concentration in the headspace dropped to around 1150 ppm. The sample of vinyl acetate / ethylene polymer emulsion stabilized with AIRFLEX400 polyvinyl alcohol was taken out from the container, and the concentration of liquefied gluconoacetic acid bacteria in potato dextrose agar was determined by dilution plate method, and the liquefied gluconoacetic acid in AIRFLEX400 emulsion was found Bacillus contamination concentration is 1.3×10 4 cfu / ml.

[0036] This example shows that t...

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Abstract

This invention relates to an improved process for the control of biocontamination in aqueous based polymeric emulsions contained in vessels having a headspace. The improvement comprises monitoring the carbon dioxide concentration in the headspace of the vessel using a direct reading carbon dioxide probe; and, then adding biocide when a preselected concentration of carbon dioxide above the atmospheric carbon dioxide concentration is reached.

Description

Background technique [0001] Water-based polymeric emulsions consist of fine particles of organic polymers suspended and stabilized in an aqueous environment containing surfactants or protective colloids or a mixture of both. Since this type of polymeric emulsion itself has carbon that provides nutrition for microorganisms, it is susceptible to microbial erosion and reproduction. This microbial attack and multiplication can lead to extensive biofouling and rancidity. It is standard industry practice to combat biological deterioration of products by adding various industrial antimicrobial agents. The addition of antimicrobial agents during production can prevent the polymeric emulsion from microbial contamination for a certain period of time, but during storage, microbial contamination will still occur and there will be a large number of microbial growth in contaminated products, for example, at 1×10 2 cfu / ml or more (cfu: colony forming unit), which leads to changes in its pH...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/44A61L2/16A61L2/28G01N33/00
CPCG01N33/004A61L2/28A61L2/16Y10T436/12Y10T436/204998G01N33/44
Inventor J·J·拉巴斯科R·H·博特F·K·施维哈德特D·萨格
Owner AIR PRODS POLYMERS
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