Microbial pollution source tracing method

Abstract

The invention provides a microbial pollution source tracing method. The method comprises the following steps: determining each suspicious pollution link of a polluted product, and respectively carrying out sampling to obtain a plurality of samples to be analyzed; determining corresponding culture conditions according to the environment where each sample to be analyzed is located, and culturing microorganisms in each sample to be analyzed according to the determined culture conditions to obtain corresponding purified single colonies; performing mass spectrometric analysis on each single colonyby using a matrix-assisted laser desorption ionization-time-of-flight mass spectrometer to obtain spectrogram data of each single colony and identify species information of each single colony; determining species distribution information of microorganisms in each sample to be analyzed according to the species information of each single colony; and analyzing and comparing the species distribution information of the microorganisms of the polluted product with the species distribution information of the microorganisms of each suspicious pollution link, and determining a pollution source accordingto an analysis result. According to the invention, microbial pollution source tracing can be rapidly and efficiently realized.

Description

technical field [0001] The present application relates to the technical field of traceability of pollution sources, in particular to a method for traceability of microbial pollution sources. Background technique [0002] Microbial traceability analysis is to judge the relationship between the sample and the pollution source by comparing the difference between the pollution indicator microorganisms in the contaminated sample and the pollution source or the presence or absence of biomarkers, so as to determine its possible pollution source. [0003] The determination of contamination indicator microorganisms is a key component of microbial traceability, usually by using traditional microbial isolation and culture techniques to directly detect the presence or absence of indicator microorganisms in samples, or by molecular biological methods (based on nucleic acid probes, PCR, to find There are similar but different gene maps, backtracking their hosts) and phenotypic methods (co...

AI Technical Summary

Problems solved by technology

However, since there are more than one links involved in the production process, and there are many types and quantities of contaminating bacteria involved in each link, and it is difficult to predict, it is difficult to use existing technologies for traceability analysis

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