Method for inducing human ADSC (Adipose-Derived Stem Cells) to be differentiated into LCs (Leydig Cells) by micromolecules

Abstract

The invention discloses a method for inducing human ADSC (Adipose-Derived Stem Cells) to be differentiated into LCs (Leydig Cells) by micromolecules. The method comprises the following steps: firstly,separating and extracting the human ADSC, and completing identification; then, inducing differentiation of the human ADSC by adopting a specific culture medium, and simultaneously adding a certain amount of micromolecules such as SAG, 22OHC, Li, PDGF-AA, FGF2, Androgen and LH in the special culture medium at different time points, thus promoting differentiation; finally, manually removing ALCs (Adult Leydig Cells), enriching and differentiating target cells ADSC-ALCs, and completing the identification. By inducing according to the method, the human ADSC can be successfully differentiated intothe LCs, so that a cell source is provided for treating testosterone deficiency by adopting cell transplantation.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, in particular to a method for fully utilizing small molecules to induce human adipose stem cells to differentiate into Leydig cells. Background technique [0002] Male reproductive health is inseparable from the functioning of the reproductive system. Currently, the only treatment for androgen deficiency is androgen replacement therapy. However, long-term androgen therapy can cause complications such as liver and kidney damage, decreased immunity, water and sodium retention, and is not regulated by its own circadian rhythm. At present, a hot spot in medical research is cell replacement therapy. Although Leydig cells (LCs) transplantation can avoid some complications caused by exogenous androgen replacement therapy, insufficient sources of Leydig cells (LCs) and allogeneic transplantation may lead to host immune rejection The reaction limits the clinical application of Leydig cells (LCs)....

AI Technical Summary

Problems solved by technology

After years of research, adipose stem cells (ADSCs) can be induced to differentiate into various cells, but the technology of inducing human adipose stem cells to differentiate into Leydig cells through small molecules has not been disclosed; therefore, this patent will be based on the use of human fat Stem cells (hADSCs), using small molecules instead of gene transfer methods to induce the differentiation of adipose-derived stem cells (ADSCs) into functional mature Leydig cells (ALCs), for future patients using autologous adipose-derived stem cells (ADSCs) to differentiate testicular Leydig cells ( ALC) transplantation provides scientific basis for the treatment of androgen deficiency

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